Anti-Chromogranin A antibody [LK2H10 + PHE5 + CGA/414] - BSA and Azide free (ab212295)

Overview

  • Product name

    Anti-Chromogranin A antibody [LK2H10 + PHE5 + CGA/414] - BSA and Azide free
    See all Chromogranin A primary antibodies
  • Description

    Mouse monoclonal [LK2H10 + PHE5 + CGA/414] to Chromogranin A - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Monkey
  • Immunogen

    This product was produced with the following immunogens:
    Tissue, cells or virus corresponding to Human Chromogranin A. (Human pheochromocytoma (LK2H10 & PHE5)).

    Recombinant full length protein corresponding to Human Chromogranin A. (CGA414).
    Database link: P10645

  • Positive control

    • IHC-P: Human adrenal gland and small cell lung carcinoma tissues.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

Properties

Applications

Our Abpromise guarantee covers the use of ab212295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.25 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similarities

    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications

    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links

  • Form

    According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • Alternative names

    • beta Granin antibody
    • betagranin (N-terminal fragment of chromogranin A) antibody
    • catestatin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • chromofungin antibody
    • Chromogranin A antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • Secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • vasostatin 2 antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    • vasostatin-2 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Chromogranin A using ab212295 at 0.25 µg/ml in immunohistochemical analysis.

    This IHC-P data was generated using the same anti-Chromogranin A antibody clone, LK2H10 + PHE5 + CGA/414, in a different buffer formulation (ab199014).

  • Formalin-fixed, paraffin-embedded human small cell lung carcinoma tissue stained for Chromogranin A using ab212295 at 0.25 µg/ml in immunohistochemical analysis.

    This IHC-P data was generated using the same anti-Chromogranin A antibody clone, LK2H10 + PHE5 + CGA/414, in a different buffer formulation (ab199014).

References

ab212295 has not yet been referenced specifically in any publications.

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