Anti-Chromogranin A antibody, prediluted (ab15162)

Submit a review Q&A (2)

Overview

  • Product name
    Anti-Chromogranin A antibody, prediluted
    See all Chromogranin A primary antibodies
  • Description
    Rabbit polyclonal to Chromogranin A, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Chromogranin A.
    Database link: P10645

  • Positive control
    • IHC-P: Human pancreas tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab15162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Pre-diluted / ready to use

Target

  • Function
    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similarities
    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications
    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links
    • Form
      According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • Alternative names
      • beta Granin antibody
      • betagranin (N-terminal fragment of chromogranin A) antibody
      • catestatin antibody
      • CgA antibody
      • CHG A antibody
      • Chga antibody
      • chromofungin antibody
      • Chromogranin A antibody
      • Chromogranin A parathyroid secretory protein 1 antibody
      • Chromogranin A precursor antibody
      • ChromograninA antibody
      • CMGA_HUMAN antibody
      • ER-37 antibody
      • Pancreastatin antibody
      • Parastatin antibody
      • Parathyroid secretory protein 1 antibody
      • Pituitary secretory protein I antibody
      • Secretory protein I antibody
      • SP I antibody
      • SP-I antibody
      • SP1 antibody
      • SPI antibody
      • vasostatin 2 antibody
      • Vasostatin antibody
      • Vasostatin I antibody
      • Vasostatin II antibody
      • vasostatin-2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody, prediluted (ab15162)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody, prediluted (ab15162)

      Formalin-fixed, paraffin-embedded human pancreas tissue stained for Chromogranin A using ab15162 in immunohistochemical analysis.

    References

    ab15162 has not yet been referenced specifically in any publications.

    Publishing research using ab15162? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Thanks, but none of those you suggest are affinity-purified anti-human chromogranin A pAbs.

    That is why I asked if the pre-diluted ones could be provided at a higher concentration?

    Read More
    Answer

    Unfortunately we do not sell these undiluted. I am sorry I could not be of further help in this instance.

    Read More

    Question

    Good Morning,

    I would like to order an affinity-purified anti-human chromogranin A pAg generated against the whole protein.

    I found two products (ab15162 and ab75939), but they were both pre-diluted. To what concentration? I need 1 mg/ml. Is either of the Abs available in a concentrated form?


    Thanks in advance.

    Read More
    Answer

    Thank you for contacting us.

    I dont have the exact concentration of the two pre-diluted products (ab15162 & ab75939) but it is very unlikely that the concentration will be around 1 mg/ml. This is a common concentration for undiluted antibody. I have found some other antibodies that have been raised against the full length protein that may be of interest and have details about their concentration:.

    ab16693
    www.abcam.com/ab16693
    Immunogen: Recombinant full length protein (Human)
    Tissue culture supernatant - Total antibody concentration ˜ 1 mg/ml. Specific antibody concentration ˜ 0.05 mg/ml

    ab61967
    www.abcam.com/ab61967
    Immunogen: Full length native protein (purified) (Human)
    IgY fraction - 500µl at 12.2 mg/ml

    ab91215
    www.abcam.com/ab91215
    Immunogen: Full length native protein (purified) (Human) expressed in mammalian cells
    Protein G purified - 100µg unit at 2mg/ml

    I hope this information helps. Just let me know if you have further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up