Anti-Chromogranin A antibody, prediluted (ab15162)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody, prediluted (ab15162)

    Key features and details

    • Rabbit polyclonal to Chromogranin A, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

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    Anti-Chromogranin A antibody [EP1030Y] (ab68271)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Chromogranin A antibody, prediluted
      See all Chromogranin A primary antibodies

    • Description

      Rabbit polyclonal to Chromogranin A, prediluted

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant full length protein corresponding to Human Chromogranin A.
      Database link: P10645

    • Positive control

      • IHC-P: Human pancreas tissue.

    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab15162 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Pre-diluted / ready to use
    Notes
    IHC-P
    1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Pre-diluted / ready to use

    Target

    • Function

      Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.

    • Sequence similarities

      Belongs to the chromogranin/secretogranin protein family.

    • Post-translational
      modifications

      Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.

    • Cellular localization

      Secreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt

    • Database links

      • Form

        According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.

      • Alternative names

        • beta Granin antibody
        • betagranin (N-terminal fragment of chromogranin A) antibody
        • catestatin antibody
        • CgA antibody
        • CHG A antibody
        • Chga antibody
        • chromofungin antibody
        • Chromogranin A antibody
        • Chromogranin A parathyroid secretory protein 1 antibody
        • Chromogranin A precursor antibody
        • ChromograninA antibody
        • CMGA_HUMAN antibody
        • ER-37 antibody
        • Pancreastatin antibody
        • Parastatin antibody
        • Parathyroid secretory protein 1 antibody
        • Pituitary secretory protein I antibody
        • Secretory protein I antibody
        • SP I antibody
        • SP-I antibody
        • SP1 antibody
        • SPI antibody
        • vasostatin 2 antibody
        • Vasostatin antibody
        • Vasostatin I antibody
        • Vasostatin II antibody
        • vasostatin-2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody, prediluted (ab15162)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody, prediluted (ab15162)

        Formalin-fixed, paraffin-embedded human pancreas tissue stained for Chromogranin A using ab15162 in immunohistochemical analysis.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab15162? Please let us know so that we can cite the reference in this datasheet.

      ab15162 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Thanks, but none of those you suggest are affinity-purified anti-human chromogranin A pAbs.

      That is why I asked if the pre-diluted ones could be provided at a higher concentration?

      Read More
      Answer

      Unfortunately we do not sell these undiluted. I am sorry I could not be of further help in this instance.

      Read More

      Question

      Good Morning,

      I would like to order an affinity-purified anti-human chromogranin A pAg generated against the whole protein.

      I found two products (ab15162 and ab75939), but they were both pre-diluted. To what concentration? I need 1 mg/ml. Is either of the Abs available in a concentrated form?


      Thanks in advance.

      Read More
      Answer

      Thank you for contacting us.

      I dont have the exact concentration of the two pre-diluted products (ab15162 & ab75939) but it is very unlikely that the concentration will be around 1 mg/ml. This is a common concentration for undiluted antibody. I have found some other antibodies that have been raised against the full length protein that may be of interest and have details about their concentration:.

      ab16693
      www.abcam.com/ab16693
      Immunogen: Recombinant full length protein (Human)
      Tissue culture supernatant - Total antibody concentration ˜ 1 mg/ml. Specific antibody concentration ˜ 0.05 mg/ml

      ab61967
      www.abcam.com/ab61967
      Immunogen: Full length native protein (purified) (Human)
      IgY fraction - 500µl at 12.2 mg/ml

      ab91215
      www.abcam.com/ab91215
      Immunogen: Full length native protein (purified) (Human) expressed in mammalian cells
      Protein G purified - 100µg unit at 2mg/ml

      I hope this information helps. Just let me know if you have further questions.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com