Overview

  • Product name
    Anti-Chromogranin A antibody [SP12]
    See all Chromogranin A primary antibodies
  • Description
    Rabbit monoclonal [SP12] to Chromogranin A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human Chromogranin A. The exact sequence is proprietary.

  • Positive control
    • Human pancreas

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab16693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 for 10 min at room temperature. Perform heat mediated antigen retrieval by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
    • Sequence similarities
      Belongs to the chromogranin/secretogranin protein family.
    • Post-translational
      modifications
      Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.
    • Cellular localization
      Secreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt
    • Database links
    • Form
      According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
    • Alternative names
      • beta Granin antibody
      • betagranin (N-terminal fragment of chromogranin A) antibody
      • catestatin antibody
      • CgA antibody
      • CHG A antibody
      • Chga antibody
      • chromofungin antibody
      • Chromogranin A antibody
      • Chromogranin A parathyroid secretory protein 1 antibody
      • Chromogranin A precursor antibody
      • ChromograninA antibody
      • CMGA_HUMAN antibody
      • ER-37 antibody
      • Pancreastatin antibody
      • Parastatin antibody
      • Parathyroid secretory protein 1 antibody
      • Pituitary secretory protein I antibody
      • Secretory protein I antibody
      • SP I antibody
      • SP-I antibody
      • SP1 antibody
      • SPI antibody
      • vasostatin 2 antibody
      • Vasostatin antibody
      • Vasostatin I antibody
      • Vasostatin II antibody
      • vasostatin-2 antibody
      see all

    Images

    • Paraffin embedded formalin fixed human pancreas stained with anti Chromogranin A antibody ab16693 at 1/100 dilution.

    References

    This product has been referenced in:
    • Anderson WC  et al. Initiation and characterization of small cell lung cancer patient-derived xenografts from ultrasound-guided transbronchial needle aspirates. PLoS One 10:e0125255 (2015). Read more (PubMed: 25955027) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    I dont have the exact concentration of the two pre-diluted products (ab15162 & ab75939) but it is very unlikely that the concentration will be around 1 mg/ml. This is a common concentration for undiluted antibody. I have found some other antibodies that have been raised against the full length protein that may be of interest and have details about their concentration:.

    ab16693
    www.abcam.com/ab16693
    Immunogen: Recombinant full length protein (Human)
    Tissue culture supernatant - Total antibody concentration ˜ 1 mg/ml. Specific antibody concentration ˜ 0.05 mg/ml

    ab61967
    www.abcam.com/ab61967
    Immunogen: Full length native protein (purified) (Human)
    IgY fraction - 500µl at 12.2 mg/ml

    ab91215
    www.abcam.com/ab91215
    Immunogen: Full length native protein (purified) (Human) expressed in mammalian cells
    Protein G purified - 100µg unit at 2mg/ml

    I hope this information helps. Just let me know if you have further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up