Product nameAnti-Chromogranin B antibody
See all Chromogranin B primary antibodies
DescriptionRabbit polyclonal to Chromogranin B
SpecificityAb12242 recognises chromogranin B.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, ICC, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
C-terminal 15 amino acids of chromogranin B conjugated to ovalbumin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab12242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 78.2 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24454876|
|IHC-Fr||1/100 - 1/500.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionSecretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Tissue specificityExpressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver.
Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
modificationsExtensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues.
Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
- CCB peptide antibody
- Cg B antibody
- CgB antibody
ab12242(1:1000) staining Chromogranin B in human pancreas (IgG inset) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Chromogranin B in secretory compartments of the pancreas.
Insert panel depicts negative control (no primary antibody).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX.
Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
This product has been referenced in:
- Stephens SB et al. The Prohormone VGF Regulates ß Cell Function via Insulin Secretory Granule Biogenesis. Cell Rep 20:2480-2489 (2017). WB, ICC/IF . Read more (PubMed: 28877479) »
- Wang H et al. RanBP9 overexpression accelerates loss of pre and postsynaptic proteins in the AP?E9 transgenic mouse brain. PLoS One 9:e85484 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24454876) »