Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)
Key features and details
- Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat
- Isotype: IgG2b
Overview
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Product name
Anti-Chromogranin C/SGII antibody [SGII 6B1/3]
See all Chromogranin C/SGII primary antibodies -
Description
Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII -
Host species
Mouse -
Specificity
SGII6B1/3 has potential uses for chromatogranin diagnostics. -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Rat Chromogranin C/SGII (C terminal).
Sequence:KEENSRENPF
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SGII 6B1/3 -
Myeloma
Sp2 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 24765 Rat
- SwissProt: P10362 Rat
- Unigene: 45602 Rat
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Alternative names
- CHGC antibody
- Chromogranin C antibody
- Chromogranin-C antibody
see all
Images
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Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).
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IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab20245 has been referenced in 7 publications.
- Çakmakli GY et al. Striatal Neurotransmitter Release-related Presynaptic Proteins in L-dopa Induced Dyskinesia in a Model of Parkinsonism. Noro Psikiyatr Ars 55:73-79 (2018). PubMed: 30042645
- Chen Y et al. An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells. Biophys Rep 1:34-40 (2015). PubMed: 26942217
- Berard AR et al. Differential Reovirus-Specific and Herpesvirus-Specific Activator Protein 1 Activation of Secretogranin II Leads to Altered Virus Secretion. J Virol 89:11954-64 (2015). PubMed: 26378181
- Oh JY et al. Identification of the HSPB4/TLR2/NF-?B axis in macrophage as a therapeutic target for sterile inflammation of the cornea. EMBO Mol Med : (2012). ICC/IF ; Rat . PubMed: 22359280
- Kim CH et al. Norepinephrine deficiency is caused by combined abnormal mRNA processing and defective protein trafficking of dopamine beta-hydroxylase. J Biol Chem 286:9196-204 (2011). WB . PubMed: 21209083
- Urbé S et al. Homotypic fusion of immature secretory granules during maturation in a cell-free assay. J Cell Biol 143:1831-44 (1998). PubMed: 9864358
- Dittié AS & Tooze SA Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells. Biochem J 310 ( Pt 3):777-87 (1995). PubMed: 7575410