This antibody gave a positive signal in the following tissue lysates: Human Kidney; Human Lung; Human Adrenal gland; Human Liver.
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The band observed at 64 kDa could potentially be a cleaved form of Chromogranin C due to the presence of a 27 amino acid signal peptide and 3 amino acid propeptide.
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C antibody (ab104367)
ICC/IF image of ab104367 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104367 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Chromogranin C antibody (ab104367)
All lanes : Anti-Chromogranin C antibody (ab104367) at 1 µg/ml
Lane 1 : Human adrenal normal tissue lysate - total protein (ab29249) Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution