Anti-Chromogranin C antibody [SGII 6B1/3] (ab20245)


  • Product name
    Anti-Chromogranin C antibody [SGII 6B1/3]
    See all Chromogranin C primary antibodies
  • Description
    Mouse monoclonal [SGII 6B1/3] to Chromogranin C
  • Host species
  • Specificity
    SGII6B1/3 has potential uses for chromatogranin diagnostics.
  • Tested applications
    Suitable for: IP, Flow Cyt, IHC-Fr, WB, ICC/IF, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide:





Our Abpromise guarantee covers the use of ab20245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 71 kDa.
ICC/IF Use at an assay dependent concentration.
ICC Use a concentration of 2 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides.
  • Sequence similarities
    Belongs to the chromogranin/secretogranin protein family.
  • Cellular localization
    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHGC antibody
    • Chromogranin-C antibody
    • EM66 antibody
    • SCG2 antibody
    • SCG2_HUMAN antibody
    • Secretogranin II antibody
    • Secretogranin-2 antibody
    • Secretoneurin antibody
    • SGII antibody
    • SN antibody
    see all


  • Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).

  • IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Chen Y  et al. An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells. Biophys Rep 1:34-40 (2015). Read more (PubMed: 26942217) »
  • Berard AR  et al. Differential Reovirus-Specific and Herpesvirus-Specific Activator Protein 1 Activation of Secretogranin II Leads to Altered Virus Secretion. J Virol 89:11954-64 (2015). Read more (PubMed: 26378181) »

See all 6 Publications for this product

Customer reviews and Q&As

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Of the Chromogranin C antibodies we have, ab20245 will be the best non-rabbit-host choice. It has not been tested for reactivity with mouse samples but the immunogen sequence, derived from rat protein, is 100% co...

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