Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)

Reviews (1)Q&A (1)References (7)
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)

Key features and details

  • Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Chromogranin C/SGII antibody [SGII 6B1/3]
    See all Chromogranin C/SGII primary antibodies

  • Description

    Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII

  • Host species

    Mouse

  • Specificity

    SGII6B1/3 has potential uses for chromatogranin diagnostics.

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Synthetic peptide corresponding to Rat Chromogranin C/SGII (C terminal).
    Sequence:

    KEENSRENPF

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides.

  • Sequence similarities

    Belongs to the chromogranin/secretogranin protein family.

  • Cellular localization

    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt

  • Database links

    • Alternative names

      • CHGC antibody
      • Chromogranin C antibody
      • Chromogranin-C antibody
      • EM66 antibody
      • SCG2 antibody
      • SCG2_HUMAN antibody
      • Secretogranin II antibody
      • Secretogranin-2 antibody
      • Secretoneurin antibody
      • SGII antibody
      • SN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)
      Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France

      Confocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)
      IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (7)

    Publishing research using ab20245? Please let us know so that we can cite the reference in this datasheet.

    ab20245 has been referenced in 7 publications.

    • Çakmakli GY  et al. Striatal Neurotransmitter Release-related Presynaptic Proteins in L-dopa Induced Dyskinesia in a Model of Parkinsonism. Noro Psikiyatr Ars 55:73-79 (2018). PubMed: 30042645
    • Chen Y  et al. An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells. Biophys Rep 1:34-40 (2015). PubMed: 26942217
    • Berard AR  et al. Differential Reovirus-Specific and Herpesvirus-Specific Activator Protein 1 Activation of Secretogranin II Leads to Altered Virus Secretion. J Virol 89:11954-64 (2015). PubMed: 26378181
    • Oh JY  et al. Identification of the HSPB4/TLR2/NF-?B axis in macrophage as a therapeutic target for sterile inflammation of the cornea. EMBO Mol Med : (2012). ICC/IF ; Rat . PubMed: 22359280
    • Kim CH  et al. Norepinephrine deficiency is caused by combined abnormal mRNA processing and defective protein trafficking of dopamine beta-hydroxylase. J Biol Chem 286:9196-204 (2011). WB . PubMed: 21209083
    • Urbé S  et al. Homotypic fusion of immature secretory granules during maturation in a cell-free assay. J Cell Biol 143:1831-44 (1998). PubMed: 9864358
    • Dittié AS & Tooze SA Characterization of the endopeptidase PC2 activity towards secretogranin II in stably transfected PC12 cells. Biochem J 310 ( Pt 3):777-87 (1995). PubMed: 7575410

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Chromogranin C antibody [SGII 6B1/3]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Hippocampal neuron culture)
    Specification
    Hippocampal neuron culture
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 5%
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    Dr. Randal Moldrich

    Verified customer

    Submitted May 17 2006

    Question

    I am searching for a secretogranin 2 (chromogranin C) antibody that works on mouse material in ICC application. It has to be of a non-rabbit origin. Would Anti-Chromogranin C antibody [SGII 6B1/3] (ab20245) be suitable? If not, do you have an alternative?

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    Answer

    Thank you for contacting us.

    Of the Chromogranin C antibodies we have, ab20245 will be the best non-rabbit-host choice. It has not been tested for reactivity with mouse samples but the immunogen sequence, derived from rat protein, is 100% conserved in mouse Chromogranin C. However, we do not guarantee reactivity with untested species, which would include mouse in this case.

    Please do not hesitate to contact us if you need any more advice or information.

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