Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-Chromogranin C/SGII antibody [SGII 6B1/3]
See all Chromogranin C/SGII primary antibodies -
Description
Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII -
Host species
Mouse -
Specificity
SGII6B1/3 has potential uses for chromatogranin diagnostics. -
Tested applications
Suitable for: IP, Flow Cyt, IHC-Fr, WB, ICC/IF, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Rat Chromogranin C/SGII (C terminal).
Sequence:KEENSRENPF
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General notes
Previously labelled as Chromogranin C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SGII 6B1/3 -
Myeloma
Sp2 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab20245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 71 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ICC | Use a concentration of 2 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 24765 Rat
- SwissProt: P10362 Rat
- Unigene: 45602 Rat
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Alternative names
- CHGC antibody
- Chromogranin C antibody
- Chromogranin-C antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)](https://www.abcam.com/ps/products/20/ab20245/Images/ab20245-29542-anti-chromogranin-c-sgii-antibody-sgii-6b1-3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceConfocal image of immunofluorescence staining with Mouse monoclonal [SGII 6B1/3] to Chromogranin C/SGII;(ab20245; 2µg/ml for 1h at room temperature) in red and nuclear counterstain in blue on Rat Cell (Hippocampal neuron culture). Cells were fixed with paraformaldehyde; BSA (5%) was used as a blocking agent (40 minutes at room temperature). Secondary antibody was Goat anti-mouse IgG2b Alexa Fluor 568 Conjugation (1/600).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)](https://www.abcam.com/ps/products/20/ab20245/Images/ab20245-29541-anti-chromogranin-c-sgii-antibody-sgii-6b1-3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [SGII 6B1/3] (ab20245)IHC image of ab20245 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20245, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References
This product has been referenced in:
- Çakmakli GY et al. Striatal Neurotransmitter Release-related Presynaptic Proteins in L-dopa Induced Dyskinesia in a Model of Parkinsonism. Noro Psikiyatr Ars 55:73-79 (2018). Read more (PubMed: 30042645) »
- Chen Y et al. An efficient two-step subcellular fractionation method for the enrichment of insulin granules from INS-1 cells. Biophys Rep 1:34-40 (2015). Read more (PubMed: 26942217) »
