Key features and details
- Rabbit polyclonal to CHSS2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHSS2 antibody
See all CHSS2 primary antibodies
DescriptionRabbit polyclonal to CHSS2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human small intestine tissue. WB: CHSS2 overexpression HEK-293T cell lysate. ICC/IF: U-251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 85 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
RelevanceCHSS2 belongs to the chondroitin N-acetylgalactosaminyltransferase family. It has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity and transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. CHSS2 is ubiquitous; highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta.
Cellular localizationGolgi apparatus, golgi stack membrane; Single pass type II membrane protein.
- Chondroitin glucuronyltransferase II antibody
- Chondroitin polymerizing factor antibody
- Chondroitin sulfate synthase 2 antibody
Paraffin-embedded human small intestine tissue stained for CHSS2 using ab224495 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-CHSS2 antibody (ab224495) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : CHSS2 overepression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Developed using the ECL technique.
Predicted band size: 85 kDa
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CHSS2 (green) using ab224495 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224495 has been referenced in 1 publication.
- Xu Q et al. Chondroitin polymerizing factor (CHPF) contributes to malignant proliferation and migration of hepatocellular carcinoma cells. Biochem Cell Biol 98:362-369 (2020). PubMed: 32383983