Key features and details
- Rabbit polyclonal to CHST14
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHST14 antibody
DescriptionRabbit polyclonal to CHST14
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: A549 cell lysate. IHC-P: Human pancreatic cancer tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
FunctionCatalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA.
Tissue specificityWidely expressed. Expressed at high level in pituitary gland, placenta, uterus and thyroid.
Involvement in diseaseDefects in CHST14 are the cause of Ehlers-Danlos syndrome musculocontractural type (EDSMC) [MIM:601776]. It is a form of Ehlers-Danlos syndrome characterized by distinctive craniofacial dysmorphism, congenital contractures of thumbs and fingers, clubfeet, severe kyphoscoliosis, muscular hypotonia, hyperextensible thin skin with easy bruisability and atrophic scarring, wrinkled palms, joint hypermobility, and ocular involvement.
Sequence similaritiesBelongs to the sulfotransferase 2 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 2600016L03Rik antibody
- ATCS antibody
- Carbohydrate (N acetylgalactosamine 4 0) sulfotransferase 14 antibody
Anti-CHST14 antibody (ab235056) at 1/500 dilution + A549 (human lung carcinoma cell line) cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Paraffin-embedded human pancreatic cancer tissue stained for CHST14 using ab235056 at 1/800 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235056 has not yet been referenced specifically in any publications.