Overview

  • Product name

  • Description

    Rabbit polyclonal to CHST15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human CHST15 (internal sequence). The exact sequence is proprietary.
    Database link: Q7LFX5

  • Positive control

    • WB: Raji whole cell lysate. IHC-P: Human gastric cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab229223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
  • Tissue specificity

    Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1.
  • Sequence similarities

    Belongs to the sulfotransferase 1 family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Golgi apparatus membrane. A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell RAG associated gene protein antibody
    • B cell RAG associated protein antibody
    • B-cell RAG-associated gene protein antibody
    • BRAG antibody
    • Carbohydrate sulfotransferase 15 antibody
    • Chst15 antibody
    • CHSTF_HUMAN antibody
    • GALNAC4S 6ST antibody
    • GalNAc4S-6ST antibody
    • hBRAG antibody
    • KIAA0598 antibody
    • N acetylgalactosamine 4 sulfate 6 O sulfotransferase antibody
    • N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase antibody
    • ST4S6 antibody
    see all

Images

  • Anti-CHST15 antibody (ab229223) at 1/500 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg


    7.5% SDS-PAGE gel.

  • Paraffin-embedded human gastric cancer tissue stained for CHST15 using ab229223 at 1/500 dilution in immunohistochemical analysis.

References

ab229223 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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