Key features and details
- Rabbit polyclonal to CHST6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHST6 antibody
DescriptionRabbit polyclonal to CHST6
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 57-340 of Human CHST6 (Uniprot ID: Q9GZX3).
- HeLa, 293T, A431, H1299, HepG2, MOLT4 and Raji whole cell lysates.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 44 kDa.|
FunctionSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures.
Tissue specificityExpressed in cornea. Mainly expressed in brain. Also expressed in spinal cord and trachea.
Involvement in diseaseDefects in CHST6 are the cause of macular dystrophy, corneal, 1 (MCDC1) [MIM:217800]. An ocular disease characterized by bilateral, progressive corneal opacification, and reduced corneal sensitivity. Onset occurs in the first decade, usually between ages 5 and 9. Painful attacks with photophobia, foreign body sensations, and recurrent erosions occur in most patients. The disease is due to deposition of an unsulfated keratan sulfate both within the intracellular space (within the keratocytes and endothelial cells) and in the extracellular corneal stroma. Macular corneal dystrophy is divided into the clinically indistinguishable types I, IA, and II based on analysis of the normally sulfated, or antigenic, keratan sulfate levels in serum and immunohistochemical evaluation of the cornea. Patients with types I and IA macular corneal dystrophy have undetectable serum levels of antigenic keratan sulfate, whereas those with type II macular corneal dystrophy have normal or low levels, depending on the population examined. Note=CHST6 homozygous missense mutations have been observed in patients with macular corneal dystrophy type I, while type II patients show a large deletion and replacement in the upstream region of CHST6. The only missense mutation for type II is Cys-50, which is heterozygous with a replacement in the upstream region on the other allele of CHST6.
Sequence similaritiesBelongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- C GlcNAc6ST antibody
- C-GlcNAc6ST antibody
- Carbohydate sulfotransferase 6 antibody
ab154332 has been referenced in 2 publications.
- Gouveia RM et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 121:205-219 (2017). IF ; Human . PubMed: 28092777
- Abidin FZ et al. Application of retinoic acid improves form and function of tissue engineered corneal construct. Organogenesis 11:122-36 (2015). WB . PubMed: 26496651