Overview

  • Product name
    Anti-CHST7 antibody
  • Description
    Rabbit polyclonal to CHST7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 86-323 of Human CHST7 (Uniprot ID: Q9NS84).

  • Positive control
    • NT2D1, IMR32, U87-MG and MCF7 whole cell lysate. MCF7 cells. Human hepatoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    CHST7 belongs to the sulfotransferase family. Sulfotransferases generate sulfated glycosaminoglycan (GAG) moities during chondroitin sulfate biosynthesis. They create considerable structural diversity among chondroitin sulfates by transferring sulfate with remarkable specificity for the underlying oligosaccharide substrate. This protein mainly transfers sulfate to N-acetylgalactosamine. The regulated expression of each member of the family may be an important determinant of sulfated GAGs expression and the associated function of chondroitin sulfates as regulators of many biologic processes.
  • Cellular localization
    Golgi apparatus membrane; Single-pass type II membrane protein
  • Database links
  • Alternative names
    • 6 O sulfotransferase 5 antibody
    • C6ST 2 antibody
    • C6ST2 antibody
    • Carbohydrate sulfotransferase 7 antibody
    • Chondroitin 6 sulfotransferase 2 antibody
    • CHST 7 antibody
    • CHST7 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7 antibody
    • Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 5 antibody
    • GlcNAc6ST 4 antibody
    • Gn6st-4 antibody
    • GST 5 antibody
    • N acetylglucosamine 6 O sulfotransferase 1 antibody
    see all

Images

  • All lanes : Anti-CHST7 antibody (ab154726) at 1/3000 dilution

    Lane 1 : NT2D1 whole cell lysate
    Lane 2 : IMR32 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed MCF7 cells labeling CHST7 with ab154726 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling CHST7 with ab154726 at 1/500 dilution.

References

ab154726 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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