Overview

  • Product name

    Anti-CHSY1 antibody
  • Description

    Rabbit polyclonal to CHSY1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 219-528 of Human CHSY1.

  • Positive control

    • A549 and HeLa whole cell lysate (ab150035); ES2 xenograft.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 92 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
  • Tissue specificity

    Ubiquitous, with the highest levels in placenta. Detected at low levels in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, adrenal gland, mammary gland, stomach, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the chondroitin N-acetylgalactosaminyltransferase family.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Carbohydrate synthase 1 antibody
    • Chondroitin glucuronyltransferase 1 antibody
    • Chondroitin glucuronyltransferase II antibody
    • Chondroitin sulfate synthase 1 antibody
    • Chondroitin synthase 1 antibody
    • CHSS1_HUMAN antibody
    • CHSY antibody
    • ChSy-1 antibody
    • Chsy1 antibody
    • CSS1 antibody
    • Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1 antibody
    • N acetylgalactosaminyltransferase II antibody
    • N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1 antibody
    • N-acetylgalactosaminyltransferase 1 antibody
    • TPBS antibody
    see all

Images

  • Anti-CHSY1 antibody (ab153813) at 1/500 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 92 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded ES2 xenograft labeling CHSY1 with ab153813 at 1/100 dilution.

References

This product has been referenced in:

  • Zeng L  et al. CHSY1 promoted proliferation and suppressed apoptosis in colorectal cancer through regulation of the NF?B and/or caspase-3/7 signaling pathway. Oncol Lett 16:6140-6146 (2018). Read more (PubMed: 30344756) »
See 1 Publication for this product

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