Anti-ChT1 antibody (ab154493)
Key features and details
- Rabbit polyclonal to ChT1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ChT1 antibody
See all ChT1 primary antibodies -
Description
Rabbit polyclonal to ChT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
A recombinant fragment corresponding to a region within amino acids 1-212 of Human ChT1 (Uniprot ID Q86XK7).
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Positive control
- NT2D1 cell lysate; Human breast carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 42 kDa.
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| IHC-P |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 42 kDa. |
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IHC-P
1/100 - 1/1000. |
Target
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Tissue specificity
Detected only in stomach mucosa and testis, and to a much lesser level in pancreas (at protein level). Detected in gastric cancers (31%), esophageal carcinomas (50%) and ovarian cancers (23%). -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsHighly N-glycosylated. Appears not to contain significant amounts of O-linked carbohydrates or sialic acid in its sugar moieties. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 340547 Human
- Omim: 300620 Human
- SwissProt: Q86XK7 Human
- Unigene: 177164 Human
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Alternative names
- 1700062D20Rik antibody
- Cell surface A33 antigen antibody
- ChT1 thymocyte antigen antibody
see all
Images
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Western blot - Anti-ChT1 antibody (ab154493)Anti-ChT1 antibody (ab154493) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 42 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ChT1 antibody (ab154493)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma, labeling ChT1 using ab154493 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154493 has not yet been referenced specifically in any publications.