Recombinant
RabMAb

Anti-ChT1 antibody [EPR9356] (ab154186)

Overview

  • Product name
    Anti-ChT1 antibody [EPR9356]
    See all ChT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9356] to ChT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ChT1 (UniProt ID: Q86XK7).

  • Positive control
    • Human stomach, Human oesophagus cancer cell, Human ovary cancer and BxPC-3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9356
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Tissue specificity
      Detected only in stomach mucosa and testis, and to a much lesser level in pancreas (at protein level). Detected in gastric cancers (31%), esophageal carcinomas (50%) and ovarian cancers (23%).
    • Sequence similarities
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Highly N-glycosylated. Appears not to contain significant amounts of O-linked carbohydrates or sialic acid in its sugar moieties.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1700062D20Rik antibody
      • Cell surface A33 antigen antibody
      • ChT1 thymocyte antigen antibody
      • dJ889N15.1 antibody
      • Glycoprotein A34 antibody
      • GPA34 antibody
      • MGC44287 antibody
      • OTTHUMP00000023820 antibody
      • V set and immunoglobulin domain containing antibody
      • V-set and immunoglobulin domain containing 1 antibody
      • V-set and immunoglobulin domain-containing protein 1 antibody
      • VSIG1 antibody
      • VSIG1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-ChT1 antibody [EPR9356] (ab154186) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human esophagus cancer lysate
      Lane 3 : Human ovary cancer lysate
      Lane 4 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa

    References

    ab154186 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Sample
    Dog Tissue sections (Atria)
    Specification
    Atria
    Permeabilization
    Yes - 0.3% Triton X-100, 10mg/mL BSA, 5% Normal Goat Serum, in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Juan Estrada

    Verified customer

    Submitted Jul 29 2014

    Application
    Western blot
    Loading amount
    8 µg
    Gel Running Conditions
    Reduced Denaturing (4% stacking, 7.5% resolving)
    Sample
    Dog Tissue lysate - whole (atria)
    Specification
    atria
    Blocking step
    KPL Signa Lock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up