Product nameAnti-CHX10 antibody
See all CHX10 primary antibodies
DescriptionSheep polyclonal to CHX10
Tested applicationsSuitable for: IHC-Fr, WB, ChIP, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Chicken, Human
Recombinant fragment corresponding to Human CHX10 aa 1-131 (N terminal).
- WB: Rat or mouse retinal tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab16142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa. Detects a band of approximately 46 kDa mouse and rat retinal tissue lysates.|
|ChIP||Use at an assay dependent concentration. PubMed: 18272592|
|IHC-P||Use at an assay dependent concentration. PubMed: 20459797|
FunctionPlays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells.
Tissue specificityAbundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.
Involvement in diseaseDefects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI) [MIM:610092].
Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3) [MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 CVC domain.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- C elegans ceh 10 homeo domain containing homolog antibody
- Ceh 10 homeo domain containing homolog (C. elegans) antibody
- Ceh 10 homeo domain containing homolog antibody
Western blot analysis using ab161412 at 1 µg/ml on rat liver (A), rat retina tissue lysate (B), mouse liver (C) and mouse retina tissue lysate (D).
ab16142 has been referenced in 3 publications.
- Moon KH et al. Differential Expression of NF2 in Neuroepithelial Compartments Is Necessary for Mammalian Eye Development. Dev Cell 44:13-28.e3 (2018). PubMed: 29249622
- Rapicavoli NA et al. The long noncoding RNA RNCR2 directs mouse retinal cell specification. BMC Dev Biol 10:49 (2010). IHC-P ; Mouse . PubMed: 20459797
- Bharti K et al. Alternative promoter use in eye development: the complex role and regulation of the transcription factor MITF. Development 135:1169-78 (2008). ChIP ; Mouse . PubMed: 18272592