Overview

  • Product name

    Anti-CHX10 antibody [EPR7022]
    See all CHX10 primary antibodies
  • Description

    Rabbit monoclonal [EPR7022] to CHX10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CHX10 aa 300-400 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Y79 cells and cell lysate, Fetal liver lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
Flow Cyt 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Plays a significant role in the specification and morphogenesis of the sensory retina. May also participate in the development of the cells of the inner nuclear layer, particularly bipolar cells.
    • Tissue specificity

      Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.
    • Involvement in disease

      Defects in VSX2 are the cause of microphthalmia isolated type 2 (MCOP2) [MIM:610093]; also known as isolated clinical anophthalmia. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
      Defects in VSX2 are the cause of microphthalmia with cataracts and iris abnormalities (MCOPCTI) [MIM:610092].
      Defects in VSX2 are the cause of microphthalmia isolated with coloboma type 3 (MCOPCB3) [MIM:610092]; also known as isolated colobomatous microphthalmia 3. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    • Sequence similarities

      Belongs to the paired homeobox family.
      Contains 1 CVC domain.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C elegans ceh 10 homeo domain containing homolog antibody
      • Ceh 10 homeo domain containing homolog (C. elegans) antibody
      • Ceh 10 homeo domain containing homolog antibody
      • Ceh 10 homeodomain containing homolog antibody
      • Ceh 10 homeodomain containing homolog (C. elegans) antibody
      • Ceh-10 homeodomain-containing homolog antibody
      • Ceh10 homeo domain containing homolog antibody
      • Ceh10 homeodomain containing homolog antibody
      • CHX 10 antibody
      • Homeobox protein CHX 10 antibody
      • Homeobox protein CHX10 antibody
      • HOX 10 antibody
      • HOX10 antibody
      • MCOP 2 antibody
      • MCOP2 antibody
      • MCOPCB 3 antibody
      • MCOPCB3 antibody
      • RET 1 antibody
      • RET1 antibody
      • Visual system homeobox 2 antibody
      • Vsx 2 antibody
      • Vsx2 antibody
      • VSX2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-CHX10 antibody [EPR7022] (ab133636) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 39 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of permeabilized Y79 cells labelling CHX10 with ab133636 at 1/500.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133636.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up