Anti-Chymotrypsin antibody [4E1] (ab35694)

Submit a review Q&A (1)References (5)

Overview

  • Product name

    Anti-Chymotrypsin antibody [4E1]
    See all Chymotrypsin primary antibodies

  • Description

    Mouse monoclonal [4E1] to Chymotrypsin

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IHC-Fr, ELISA, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length native pancreatic protein (purified) (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab35694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/800.
ELISA 1/20000.
WB 1/400 - 1/4000. Predicted molecular weight: 29 kDa.

Target

  • Function

    Has chymotrypsin-type protease activity and hypocalcemic activity.

  • Tissue specificity

    Pancreas.

  • Involvement in disease

    Variations in CTRC influence susceptibility to chronic pancreatitis [MIM:167800]. Chronic pancreatitis is a persistent inflammatory disorder characterized by permanent destruction of the pancreatic parenchyma.

  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Caldecrin antibody
    • Chymotrypsin C antibody
    • Chymotrypsin-C antibody
    • ChymotrypsinC antibody
    • CLCR antibody
    • Ctrc antibody
    • CTRC_HUMAN antibody
    • ELA 4 antibody
    • ELA4 antibody
    • Elastase 4 antibody
    • Elastase IV antibody
    • Elastase4 antibody
    • ElastaseIV antibody
    • OTTHUMP00000044526 antibody
    • Serum calcium decreasing factor antibody
    see all

References

This product has been referenced in:

  • Song Y  et al. Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma. Transl Oncol 11:691-699 (2018). WB ; Human . Read more (PubMed: 29631213) »
  • Hong X  et al. Challenges in detecting pre-malignant pancreatic lesions during acute pancreatitis using a serum microRNA assay: a study based on KrasG12D transgenic mice. Oncotarget 7:22700-10 (2016). Read more (PubMed: 27009811) »
See all 5 Publications for this product

Publishing research using ab35694? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Submit a question

Question

  I have a question with regard to some of your anti-chymotrypsin antibodies (ab35694 and ab118845). Have you ever evaluated if these antibodies bind to porcine chymotrypsin?  

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Answer

Thank you for contacting Abcam regarding our anti-chymotrypsin antibodies. I have compared the human and porcine chemotrypsin sequences and overall, they share 84% identity.  Unfortunately, we have not mapped the epitopes recognized by these antibodies, so it is not possible to know if the regions of homology correlate to the sequences recognized by these antibodies. I am happy to offer a testing discount whereby you order one or both of these antibodies, test them with your porcine samples and submit your results in the form of an Abreview.  Once the review(s) has been submitted, the discount codes I will provide will become active and can be used towards a free primary antibody on your next purchase from us.  Please reply to this email and I will issue the code(s). I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

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