Chymotrypsin Assay Kit (Fluorometric) (ab234051)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
Overview
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Product name
Chymotrypsin Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Chymotrypsin Assay Kit (Fluorometric) (ab234051) uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore. The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K352 Chymotrypsin Activity Assay Kit (Fluorometric). K352-100 is the same size as the 100 test size of ab234051.
Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMC Standard 1 x 100µl Assay Buffer I 1 x 25ml Chymotrypsin Activator 1 vial Chymotrypsin Inhibitor I 1 x 80µl Chymotrypsin Positive Control 1 vial Chymotrypsin Substrate 1 x 200µl -
Research areas
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Function
Has chymotrypsin-type protease activity and hypocalcemic activity. -
Tissue specificity
Pancreas. -
Involvement in disease
Variations in CTRC influence susceptibility to chronic pancreatitis [MIM:167800]. Chronic pancreatitis is a persistent inflammatory disorder characterized by permanent destruction of the pancreatic parenchyma. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- Caldecrin
- Chymotrypsin C
- Chymotrypsin-C
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab234051 has been referenced in 1 publication.
- Huang H et al. Transgenic Expression of PRSS1R122H Sensitizes Mice to Pancreatitis. Gastroenterology 158:1072-1082.e7 (2020). PubMed: 31419436