Overview

  • Product name

    Chymotrypsin Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Tissue Lysate
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Chymotrypsin Assay Kit (Fluorometric) (ab234051) uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore. The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.

  • Notes

    Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine).

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Chymotrypsin Assay Buffer 1 x 25ml
    Chymotrypsin Substrate 1 x 200µl
    Chymotrypsin Activator 1 vial
    Chymotrypsin Inhibitor 1 x 80µl
    Coumarin Standard (1 mM) 1 x 100µl
    Chymotrypsin Positive Control 1 vial
  • Research areas

  • Function

    Has chymotrypsin-type protease activity and hypocalcemic activity.
  • Tissue specificity

    Pancreas.
  • Involvement in disease

    Variations in CTRC influence susceptibility to chronic pancreatitis [MIM:167800]. Chronic pancreatitis is a persistent inflammatory disorder characterized by permanent destruction of the pancreatic parenchyma.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Alternative names

    • Caldecrin
    • Chymotrypsin C
    • Chymotrypsin-C
    • ChymotrypsinC
    • CLCR
    • Ctrc
    • CTRC_HUMAN
    • ELA 4
    • ELA4
    • Elastase 4
    • Elastase IV
    • Elastase4
    • ElastaseIV
    • OTTHUMP00000044526
    • Serum calcium decreasing factor
    see all

Images

  • The presence of non-specific chymotrypsin-like proteases in spleen leads to some activity in presence of the selective chymotrypsin inhibitor.

     

  • The substrate is cleaved by chymotrypsin but not trypsin, making the assay free from trypsin interference.

     

Protocols

References

ab234051 has not yet been referenced specifically in any publications.

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