Key features and details
- Rabbit polyclonal to CIA
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CIA antibody
See all CIA primary antibodies
DescriptionRabbit polyclonal to CIA
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide within Human CIA aa 1-50 (N terminal). The exact sequence is proprietary.
- HeLa and 293T whole cell lysates.
Protein previously labeled as NCOA5.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70831 was affinity purified using an epitope specific to CIA immobilized on solid support.
Our Abpromise guarantee covers the use of ab70831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
RelevanceCIA is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c myc in response to 17 beta estradiol (E2).
- bA465L10.6 antibody
- CIA antibody
- Coactivator independent of AF 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CIA with ab70831 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CIA antibody (ab70831) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 3 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 3 minutes
Detection of CIA by Western Blot of Immunprecipitate.
ab70831 at 1µg/ml staining CIA in HeLa whole cell lysate immunoprecipitated using ab70831 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab70831 has been referenced in 2 publications.
- Wu Y et al. Association between expression of nuclear receptor co-activator 5 protein and prognosis in postoperative patients with osteosarcoma. Oncol Lett 15:1888-1892 (2018). PubMed: 29434886
- Liu X et al. A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation. Cancer Lett 391:152-161 (2017). PubMed: 28137631