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Synthetic peptide within Human CIA aa 1-50 (N terminal). The exact sequence is proprietary.
Protein previously labeled as NCOA5.
Our Abpromise guarantee covers the use of ab70831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CIA with ab70831 at 1/200 (1µg/ml). Detection: DAB.
Detection of CIA by Western Blot of Immunprecipitate.
ab70831 at 1µg/ml staining CIA in HeLa whole cell lysate immunoprecipitated using ab70831 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab70831 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"