Overview

  • Product name

  • Description

    Rabbit polyclonal to CIA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human CIA aa 1-50 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Protein previously labeled as NCOA5.

Properties

Applications

Our Abpromise guarantee covers the use of ab70831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CIA is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c myc in response to 17 beta estradiol (E2).
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • bA465L10.6 antibody
    • CIA antibody
    • Coactivator independent of AF 2 antibody
    • KIAA1637 antibody
    • MGC28864 antibody
    • NCoA 5 antibody
    • NCOA5 antibody
    • Nuclear receptor coactivator 5 antibody
    • OTTMUSP00000001057 antibody
    • RP23-61O3.13 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CIA with ab70831 at 1/200 (1µg/ml). Detection: DAB.

  • All lanes : Anti-CIA antibody (ab70831) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 3 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa


    Exposure time: 3 minutes
  • Detection of CIA by Western Blot of Immunprecipitate.
    ab70831 at 1µg/ml staining CIA in HeLa whole cell lysate immunoprecipitated using ab70831 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Wu Y  et al. Association between expression of nuclear receptor co-activator 5 protein and prognosis in postoperative patients with osteosarcoma. Oncol Lett 15:1888-1892 (2018). Read more (PubMed: 29434886) »
  • Liu X  et al. A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation. Cancer Lett 391:152-161 (2017). Read more (PubMed: 28137631) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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