Anti-cIAP1 antibody (ab2399)

Submit a review Q&A (6)References (14)
Western blot - Anti-cIAP1 antibody (ab2399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)

Key features and details

  • Rabbit polyclonal to cIAP1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-cIAP1 antibody
    See all cIAP1 primary antibodies

  • Description

    Rabbit polyclonal to cIAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. (Peptide available as ab38272.)

  • Positive control

    • WB: Jurkat cell lysate, PC12 cell lysate, rat kidney tissue lysate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).
Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).

Target

  • Function

    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).

  • Tissue specificity

    Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

  • Sequence similarities

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • API 1 antibody
      • API1 antibody
      • Apoptosis inhibitor 1 antibody
      • Baculoviral IAP repeat containing 2 antibody
      • Baculoviral IAP repeat containing protein 2 antibody
      • Baculoviral IAP repeat-containing protein 2 antibody
      • BIRC 2 antibody
      • BIRC2 antibody
      • BIRC2_HUMAN antibody
      • C IAP1 antibody
      • C-IAP1 antibody
      • Cellular inhibitor of apoptosis 1 antibody
      • cellular inhibitor of apoptosis protein 1 antibody
      • cIAP 1 antibody
      • cIAP1 antibody
      • HIAP 2 antibody
      • HIAP-2 antibody
      • HIAP2 antibody
      • IAP 2 antibody
      • IAP homolog B antibody
      • IAP-2 antibody
      • IAP2 antibody
      • Inhibitor of apoptosis protein 2 antibody
      • MIHB antibody
      • NFR2 TRAF signalling complex protein antibody
      • RING finger protein 48 antibody
      • RNF 48 antibody
      • RNF48 antibody
      • TNFR2 TRAF signaling complex protein 2 antibody
      • TNFR2-TRAF-signaling complex protein 2 antibody
      see all

    Images

    • Western blot - Anti-cIAP1 antibody (ab2399)
      Western blot - Anti-cIAP1 antibody (ab2399)
      Anti-cIAP1 antibody (ab2399) + Jurkat Cell lysate

      Predicted band size: 69 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
      IHC image of ab2399 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (14)

    Publishing research using ab2399? Please let us know so that we can cite the reference in this datasheet.

    ab2399 has been referenced in 14 publications.

    • Craver BM  et al. The SMAC mimetic LCL-161 selectively targets JAK2V617F mutant cells. Exp Hematol Oncol 9:1 (2020). PubMed: 31908904
    • Leung DTH  et al. Combined PPAR? Activation and XIAP Inhibition as a Potential Therapeutic Strategy for Ovarian Granulosa Cell Tumors. Mol Cancer Ther 18:364-375 (2019). PubMed: 30530769
    • Cheng Z  et al. Lactobacillus raisesin vitroanticancer effect of geniposide in HSC-3 human oral squamous cell carcinoma cells. Exp Ther Med 14:4586-4594 (2017). PubMed: 29104666
    • Xu Q  et al. The cytoplasmic nuclear receptor RAR? controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun 8:425 (2017). PubMed: 28871172
    • Balakrishnan K  et al. Reactivation of Smac-mediated apoptosis in chronic lymphocytic leukemia cells: mechanistic studies of Smac mimetic. Oncotarget 7:39458-39472 (2016). PubMed: 27223062
    View all Publications for this product

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Question

    the monoclonal antibody we are using is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein.

    The polyclonalantibody we are usingis produced by immunizing animals with a synthetic peptide corresponding to a region between the first two BIR domains of c-IAP1.

    Thank you for your help.

    Sincerely yours,

    Read More
    Answer

    Thank you for your email.

    The peptide is from the sequence around amino acid 530 of human cIAP1. This sequence does not match with the immunogen sequence of polyclonal and monoclonal antibodies you have so this peptide ab38272 would be of no use.

    If you are interested in doing blocking experiment then ab38272 would block ab2399.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Question

    I would like to test the specificity ofone of our anti-cIAP1 mAb by using your cIAP1 blocking peptide.

    I would therefore know if this peptide can be used as an immunogen for other antibodies than https://www.abcam.com/cIAP1-antibody-ab2399.html.

    Thank you in advance for your kind reply.

    Sincerely yours,

    Read More
    Answer

    Thank you for contacting us.

    Could you please explain what do you mean by other antibodies? The antibodies against the same peptide should be same however difference can be seen in specificity, purity etc.

    The peptide ab38272 is from a sequence flanking 530 of human cIAP1. We unfortunately don't know the exact sequence so this peptide may not able to block your antibody.

    Do you know the immunogen sequence of your antibody?

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    samples: lysates of several human leucemic B-cells (BJAB, I-83, ...) ab2399 and ab32059: sees a band at 35 kDa, but not at 70 kDa as expected Ab: 1/50, 1/1000 overnight 4 degree regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk ab8421: sees high background and band at 55 kDa (however, band is not dominant), but all bands are of equal intensity Ab: 1/1000 and higher regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk

    Read More
    Answer

    Thank you for your phone call. I am very sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate these 3 cases and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. As we discussed, I believe these 3 products should have given satisfactory results. It appears that you may have received either faulty vials, or the shipment was affected by temperature variations, customs or other unknown factors. I apologize very much for the inconvenience, but am pleased to let you know that I have arranged for the replacement shipment as we discussed. Thank you for your understanding. I wish you good luck with your research! Please let me know if there is anything else I can help you with.

    Read More

    Question

    I know the datasheet lists the immunogen as peptide ab38272, but I was wondering if it were possible to find out the exact sequence of the peptide used as the immunogen. I understand this isn't always possible, but please let me know if it is. Than

    Read More
    Answer

    Thank you for your inquiry. We source this antibody from an outside laboratory, and unfortunately we do not have access to the exact sequence used for the immunogen. If you plan to use the antibody on a fragment of the protein, we may be able to get more specific information of whether this antibody would bind to your fragment, but unfortunately we don't have access to any further information than what is on the datasheet. I apologize that I cannot be more help

    Read More

    Question

    We would like to know which region of cIAP1 does the antibody recognize (i.e. immunogen). Thanks!

    Read More
    Answer

    Thank you for your enquiry. The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    i want to use it for immunohistochemistry, and have no idea whether it will work or not, does anybody used to try it on IHC?

    Read More
    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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