Anti-cIAP1 antibody (ab2399)

Submit a review Q&A (6)References (11)

Overview

  • Product name
    Anti-cIAP1 antibody
    See all cIAP1 primary antibodies
  • Description
    Rabbit polyclonal to cIAP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. (Peptide available as ab38272.)

  • Positive control
    • WB: Jurkat cell lysate, PC12 cell lysate, rat kidney tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).Can be blocked with cIAP1 peptide (ab38272).

Target

  • Function
    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • Tissue specificity
    Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • API 1 antibody
    • API1 antibody
    • Apoptosis inhibitor 1 antibody
    • Baculoviral IAP repeat containing 2 antibody
    • Baculoviral IAP repeat containing protein 2 antibody
    • Baculoviral IAP repeat-containing protein 2 antibody
    • BIRC 2 antibody
    • BIRC2 antibody
    • BIRC2_HUMAN antibody
    • C IAP1 antibody
    • C-IAP1 antibody
    • cIAP 1 antibody
    • cIAP1 antibody
    • HIAP 2 antibody
    • HIAP-2 antibody
    • HIAP2 antibody
    • IAP 2 antibody
    • IAP homolog B antibody
    • IAP-2 antibody
    • IAP2 antibody
    • Inhibitor of apoptosis protein 2 antibody
    • MIHB antibody
    • NFR2 TRAF signalling complex protein antibody
    • RING finger protein 48 antibody
    • RNF 48 antibody
    • RNF48 antibody
    • TNFR2 TRAF signaling complex protein 2 antibody
    • TNFR2-TRAF-signaling complex protein 2 antibody
    see all

Images

  • Western blot - Anti-cIAP1 antibody (ab2399)
    Western blot - Anti-cIAP1 antibody (ab2399)
    Anti-cIAP1 antibody (ab2399) + Jurkat Cell lysate

    Predicted band size: 69 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (ab2399)
    IHC image of ab2399 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Cheng Z  et al. Lactobacillus raisesin vitroanticancer effect of geniposide in HSC-3 human oral squamous cell carcinoma cells. Exp Ther Med 14:4586-4594 (2017). Read more (PubMed: 29104666) »
  • Xu Q  et al. The cytoplasmic nuclear receptor RAR? controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun 8:425 (2017). Read more (PubMed: 28871172) »
See all 11 Publications for this product

Publishing research using ab2399? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

the monoclonal antibody we are using is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein.

The polyclonalantibody we are usingis produced by immunizing animals with a synthetic peptide corresponding to a region between the first two BIR domains of c-IAP1.

Thank you for your help.

Sincerely yours,

Read More
Answer

Thank you for your email.

The peptide is from the sequence around amino acid 530 of human cIAP1. This sequence does not match with the immunogen sequence of polyclonal and monoclonal antibodies you have so this peptide ab38272 would be of no use.

If you are interested in doing blocking experiment then ab38272 would block ab2399.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

Read More

Question

I would like to test the specificity ofone of our anti-cIAP1 mAb by using your cIAP1 blocking peptide.

I would therefore know if this peptide can be used as an immunogen for other antibodies than https://www.abcam.com/index.html?datasheet=2399.

Thank you in advance for your kind reply.

Sincerely yours,

Read More
Answer

Thank you for contacting us.

Could you please explain what do you mean by other antibodies? The antibodies against the same peptide should be same however difference can be seen in specificity, purity etc.

The peptide ab38272 is from a sequence flanking 530 of human cIAP1. We unfortunately don't know the exact sequence so this peptide may not able to block your antibody.

Do you know the immunogen sequence of your antibody?

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Question

samples: lysates of several human leucemic B-cells (BJAB, I-83, ...) ab2399 and ab32059: sees a band at 35 kDa, but not at 70 kDa as expected Ab: 1/50, 1/1000 overnight 4 degree regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk ab8421: sees high background and band at 55 kDa (however, band is not dominant), but all bands are of equal intensity Ab: 1/1000 and higher regular PAGE system and Millipore's snap-system - same results secondary: OK block: 5% milk

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Answer

Thank you for your phone call. I am very sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate these 3 cases and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. As we discussed, I believe these 3 products should have given satisfactory results. It appears that you may have received either faulty vials, or the shipment was affected by temperature variations, customs or other unknown factors. I apologize very much for the inconvenience, but am pleased to let you know that I have arranged for the replacement shipment as we discussed. Thank you for your understanding. I wish you good luck with your research! Please let me know if there is anything else I can help you with.

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Question

I know the datasheet lists the immunogen as peptide ab38272, but I was wondering if it were possible to find out the exact sequence of the peptide used as the immunogen. I understand this isn't always possible, but please let me know if it is. Than

Read More
Answer

Thank you for your inquiry. We source this antibody from an outside laboratory, and unfortunately we do not have access to the exact sequence used for the immunogen. If you plan to use the antibody on a fragment of the protein, we may be able to get more specific information of whether this antibody would bind to your fragment, but unfortunately we don't have access to any further information than what is on the datasheet. I apologize that I cannot be more help

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Question

We would like to know which region of cIAP1 does the antibody recognize (i.e. immunogen). Thanks!

Read More
Answer

Thank you for your enquiry. The immunogen sequence of Rabbit polyclonal to cIAP1 (ab2399) was a peptide sequence flanking amino acid 530 of human cIAP1. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Question

i want to use it for immunohistochemistry, and have no idea whether it will work or not, does anybody used to try it on IHC?

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Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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