Anti-cIAP1 antibody [EPR4673] (ab108361)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-cIAP1 antibody [EPR4673]
See all cIAP1 primary antibodies -
DescriptionRabbit monoclonal [EPR4673] to cIAP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to a region within Human cIAP1.
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Positive control
- WB: Jurkat, HeLa, HT-29, and HepG2 whole cell lysate (ab7900) IHC-P: Human spleen tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR4673
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For purification use at 1/50 - 1/100 |
Target
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FunctionApoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
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Tissue specificityPresent in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
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Sequence similaritiesBelongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 329 Human
- Omim: 601712 Human
- SwissProt: Q13490 Human
- Unigene: 696238 Human
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Alternative names
- API 1 antibody
- API1 antibody
- Apoptosis inhibitor 1 antibody
see all
Images
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![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-269752-ab108361KOWB.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: cIAP1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108361 was shown to specifically react with cIAP1 when cIAP1 knockout samples were used. Wild-type and cIAP1 knockout samples were subjected to SDS-PAGE. Ab108361 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-4-ab108361IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling cIAP1 with Purified ab108361 at 1:500 dilution (2.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-5-ab108361WB1.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/2000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 1/15 dilution
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/cIAP1-Primary-antibodies-ab108361-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)ab108361, at 1/50, staining cIAP1 in paraffin-embedded Human spleen tissue by Immunohistochemistry. -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/cIAP1-Primary-antibodies-ab108361-1.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Protocols
References
This product has been referenced in:
- Lukosiute-Urboniene A et al. Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer. World J Gastroenterol 25:205-219 (2019). Read more (PubMed: 30670910) »
- Ding Y et al. Bufalin induces apoptosis in human esophageal carcinoma ECA109 cells by inhibiting the activation of the mTOR/p70S6K pathway. Oncol Lett 15:9339-9346 (2018). Read more (PubMed: 29805658) »
