Anti-cIAP1 antibody [EPR4673] (ab108361)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-cIAP1 antibody [EPR4673]
See all cIAP1 primary antibodies -
DescriptionRabbit monoclonal [EPR4673] to cIAP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to a region within Human cIAP1.
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Positive control
- Jurkat, HeLa, HT-29, and HepG2 cell lysates Paraffin-embedded Human spleen tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4673
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/50 - 1/100. |
Target
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FunctionApoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
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Tissue specificityPresent in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.
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Sequence similaritiesBelongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 329 Human
- Omim: 601712 Human
- SwissProt: Q13490 Human
- Unigene: 696238 Human
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Alternative names
- API 1 antibody
- API1 antibody
- Apoptosis inhibitor 1 antibody
see all
Images
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![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-269752-ab108361KOWB.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: cIAP1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108361 was shown to specifically react with cIAP1 when cIAP1 knockout samples were used. Wild-type and cIAP1 knockout samples were subjected to SDS-PAGE. Ab108361 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/cIAP1-Primary-antibodies-ab108361-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)ab108361, at 1/50, staining cIAP1 in paraffin-embedded Human spleen tissue by Immunohistochemistry. -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/cIAP1-Primary-antibodies-ab108361-1.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Protocols
References
This product has been referenced in:
- Ding Y et al. Bufalin induces apoptosis in human esophageal carcinoma ECA109 cells by inhibiting the activation of the mTOR/p70S6K pathway. Oncol Lett 15:9339-9346 (2018). Read more (PubMed: 29805658) »
- Liu Y et al. Effects of bufalin on the mTOR/p70S6K pathway and apoptosis in esophageal squamous cell carcinoma in nude mice. Int J Mol Med 40:357-366 (2017). Read more (PubMed: 28656204) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"