Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-cIAP1 antibody [EPR4673] (ab108361)

Submit a review Q&A (1)References (11)

Overview

  • Product name

    Anti-cIAP1 antibody [EPR4673]
    See all cIAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4673] to cIAP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to a region within Human cIAP1.

  • Positive control

    • WB: Jurkat, HeLa, HT-29, and HepG2 whole cell lysate (ab7900) IHC-P: Human spleen tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For purification use at 1/50 - 1/100
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).

    • Tissue specificity

      Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

    • Sequence similarities

      Belongs to the IAP family.
      Contains 3 BIR repeats.
      Contains 1 CARD domain.
      Contains 1 RING-type zinc finger.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • API 1 antibody
        • API1 antibody
        • Apoptosis inhibitor 1 antibody
        • Baculoviral IAP repeat containing 2 antibody
        • Baculoviral IAP repeat containing protein 2 antibody
        • Baculoviral IAP repeat-containing protein 2 antibody
        • BIRC 2 antibody
        • BIRC2 antibody
        • BIRC2_HUMAN antibody
        • C IAP1 antibody
        • C-IAP1 antibody
        • Cellular inhibitor of apoptosis 1 antibody
        • cellular inhibitor of apoptosis protein 1 antibody
        • cIAP 1 antibody
        • cIAP1 antibody
        • HIAP 2 antibody
        • HIAP-2 antibody
        • HIAP2 antibody
        • IAP 2 antibody
        • IAP homolog B antibody
        • IAP-2 antibody
        • IAP2 antibody
        • Inhibitor of apoptosis protein 2 antibody
        • MIHB antibody
        • NFR2 TRAF signalling complex protein antibody
        • RING finger protein 48 antibody
        • RNF 48 antibody
        • RNF48 antibody
        • TNFR2 TRAF signaling complex protein 2 antibody
        • TNFR2-TRAF-signaling complex protein 2 antibody
        see all

      Images

      • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: cIAP1 knockout HAP1 cell lysate (20 µg)
        Lane 3: HeLa cell lysate (20 µg)
        Lane 4: Jurkat cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab108361 was shown to specifically react with cIAP1 when cIAP1 knockout samples were used. Wild-type and cIAP1 knockout samples were subjected to SDS-PAGE.  Ab108361 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling cIAP1 with Purified ab108361 at 1:500 dilution (2.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/2000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 1/15 dilution

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

        Predicted band size: 70 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)

        ab108361, at 1/50, staining cIAP1 in paraffin-embedded Human spleen tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)
        Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)
        All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : HT29 cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 70 kDa
        Observed band size: 70 kDa

      References

      This product has been referenced in:

      • Lukosiute-Urboniene A  et al. Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer. World J Gastroenterol 25:205-219 (2019). Read more (PubMed: 30670910) »
      • Chen J  et al. SM-164 enhances the antitumor activity of adriamycin in human U2-OS cells via downregulation of X-linked inhibitor of apoptosis protein. Mol Med Rep 19:5079-5086 (2019). Read more (PubMed: 31059038) »
      See all 11 Publications for this product

      Publishing research using ab108361? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Submit a review Submit a question

      Question

      Would it be possible to book 2 vials of anti-cIAP1 ab108361 lot GR106941-1?

      I will order 1 vial to test its performances (comparison with lot GRA 42211-4 that we have already).

      Or, is it maybe a test that you do before releasing a new lot?

      Then, if lot GR106941-1 performs as expected I will order the second vial.

      Thanks to let me know about availability.

      Regards,

      Read More
      Answer

      Thank you for contacting us.

      The batch to batch variations has not been known for this antibody so all the lots will be producing similar results. Please feel free to buy any lot.

      We unfortunately will not be able to hold the lots however you can delay the shipment of one vial for 1-2 weeks. Please note that the delivery charges will be charged for both orders.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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