Recombinant Anti-cIAP1 antibody [EPR4673] (ab108361)
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-397483-anti-ciap1-antibody-epr4673-western-blot-hela-lysates.jpg "Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4673] to cIAP1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-cIAP1 antibody [EPR4673]
See all cIAP1 primary antibodies -
Description
Rabbit monoclonal [EPR4673] to cIAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, HeLa, HT-29, and HepG2 whole cell lysate (ab7900) IHC-P: Human spleen tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4673 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For purification use at 1/50 - 1/100 |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For purification use at 1/50 - 1/100 |
Target
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Function
Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). -
Tissue specificity
Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes. -
Sequence similarities
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 329 Human
- Omim: 601712 Human
- SwissProt: Q13490 Human
- Unigene: 696238 Human
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Alternative names
- API 1 antibody
- API1 antibody
- Apoptosis inhibitor 1 antibody
see all
Images
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Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BIRC2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDaLanes 1- 2: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108361 was shown to react with cIAP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265896 (knockout cell lysate ab257372) was used. Wild-type HeLa and BIRC2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108361 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-318891-anti-ciap1-antibody-epr4673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling cIAP1 with Purified ab108361 at 1:500 dilution (2.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-269752-anti-ciap1-antibody-epr4673-western-blot.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: cIAP1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.ab108361 was shown to specifically react with cIAP1 when cIAP1 knockout samples were used. Wild-type and cIAP1 knockout samples were subjected to SDS-PAGE. Ab108361 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-318919-anti-ciap1-antibody-epr4673-western-blot.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/2000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 1/15 dilution
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution
Predicted band size: 70 kDa -
![Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-120993-anti-ciap1-antibody-epr4673-western-blot.jpg)
Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)All lanes : Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-120992-anti-ciap1-antibody-epr4673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)ab108361, at 1/50, staining cIAP1 in paraffin-embedded Human spleen tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-cIAP1 antibody [EPR4673] (ab108361)](https://www.abcam.com/ps/products/108/ab108361/Images/ab108361-7-benefits-of-recombinant-antibodies.png)
Anti-cIAP1 antibody [EPR4673] (ab108361)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab108361 has been referenced in 21 publications.
- Ren Y et al. Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis. Front Pharmacol 12:560903 (2021). PubMed: 33841136
- Zhang X et al. Exploring the five different genes associated with PKCa in bladder cancer based on gene expression microarray. J Cell Mol Med 25:1759-1770 (2021). PubMed: 33452764
- Müller I et al. Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28. Mol Cell 77:970-984.e7 (2020). PubMed: 31982308
- LingHu HR et al. Bufalin Induces Glioma Cell Death by Apoptosis or Necroptosis. Onco Targets Ther 13:4767-4778 (2020). PubMed: 32581545
- Fichtner M et al. Molecular subtype-specific responses of colon cancer cells to the SMAC mimetic Birinapant. Cell Death Dis 11:1020 (2020). PubMed: 33257690