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    ciap2-antibody-ab23423.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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Anti-cIAP2 antibody (ab23423)

  • Datasheet
  • SDS
Reviews (7)Q&A (2)References (6)

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Immunocytochemistry/ Immunofluorescence - Anti-cIAP2 antibody (ab23423)
  • Western blot - Anti-cIAP2 antibody (ab23423)
  • Immunocytochemistry/ Immunofluorescence - Anti-cIAP2 antibody (ab23423)
  • Western blot - Anti-cIAP2 antibody (ab23423)

Key features and details

  • Rabbit polyclonal to cIAP2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-cIAP1 antibody [EPR4673] (ab108361)
Protein
Product image
Recombinant Human cIAP2 protein (ab167951)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-cIAP2 antibody
    See all cIAP2 primary antibodies
  • Description

    Rabbit polyclonal to cIAP2
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 85% homology with cIAP1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human cIAP2 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab25892)

  • Positive control

    • This antibody gave a positive signal in HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Necroptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human cIAP2 protein (ab167951)
  • Related Products

    • TMCB, Selective CK2 inhibitor (ab120289)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab23423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Chicken
Dog
Application Abreviews Notes
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 68 kDa).
ICC/IF (1)
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 68 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • Tissue specificity

    Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
  • Involvement in disease

    Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q13489 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 330 Human
    • Entrez Gene: 11796 Mouse
    • Entrez Gene: 78971 Rat
    • Omim: 601721 Human
    • SwissProt: Q13489 Human
    • SwissProt: O08863 Mouse
    • Unigene: 127799 Human
    • Unigene: 2026 Mouse
    • Unigene: 64578 Rat
    see all
  • Alternative names

    • AIP 1 antibody
    • AIP1 antibody
    • API 2 antibody
    • API2 antibody
    • Apoptosis inhibitor 2 antibody
    • Baculoviral IAP repeat containing 3 antibody
    • Baculoviral IAP repeat containing protein 3 antibody
    • Baculoviral IAP repeat-containing protein 3 antibody
    • BIRC 3 antibody
    • BIRC3 antibody
    • BIRC3_HUMAN antibody
    • C IAP2 antibody
    • C-IAP2 antibody
    • Cellular inhibitor of apoptosis 2 antibody
    • cellular inhibitor of apoptosis protein 2 antibody
    • CIAP 2 antibody
    • CIAP2 antibody
    • HAIP 1 antibody
    • HAIP1 antibody
    • HIAP 1 antibody
    • HIAP-1 antibody
    • HIAP1 antibody
    • IAP homolog C antibody
    • IAP-1 antibody
    • Inhibitor of apoptosis protein 1 antibody
    • MALT 2 antibody
    • MALT2 antibody
    • Mammalian IAP homolog C antibody
    • MIHC antibody
    • RING finger protein 49 antibody
    • RNF49 antibody
    • TNFR2 TRAF signaling complex protein 1 antibody
    • TNFR2 TRAF signalling complex protein antibody
    • TNFR2-TRAF-signaling complex protein 1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-cIAP2 antibody (ab23423)
    Immunocytochemistry/ Immunofluorescence - Anti-cIAP2 antibody (ab23423)

    ICC/IF image of ab23423 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23423, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Western blot - Anti-cIAP2 antibody (ab23423)
    Western blot - Anti-cIAP2 antibody (ab23423)
    All lanes : Anti-cIAP2 antibody (ab23423) at 1 µg/ml

    Lane 1 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 150 kDa, 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    A doublet was observed in Daudi, Ramos and Raji whole cell lysates (lanes 1, 4 and 5). This data suggests that ab23423 might react with both cIAP1 and cIAP2 proteins. The predicted molecular weights of cIAP1 and cIAP2 are 69-kDa and 68-kDa respectively. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-cIAP2 antibody (ab23423)
    Immunocytochemistry/ Immunofluorescence - Anti-cIAP2 antibody (ab23423)
    ab23423 staining cIAP2 in HeLa cells treated with TMCB (ab120289), by ICC/IF. Decrease in cIAP2 expression correlates with increased concentration of TMCB, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120289 (TMCB) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab23423 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Western blot - Anti-cIAP2 antibody (ab23423)
    Western blot - Anti-cIAP2 antibody (ab23423)

    HeLa cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of TMCB (ab120289). Decreased expression of cIAP2 in HeLa cells correlates with an increase in TMCB concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 40µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab23423 at 2 µg/ml and ab1801 at 2 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051 ) at 1/10000 dilution and visualised using ECL development solution.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab23423? Please let us know so that we can cite the reference in this datasheet.

    ab23423 has been referenced in 6 publications.

    • Craver BM  et al. The SMAC mimetic LCL-161 selectively targets JAK2V617F mutant cells. Exp Hematol Oncol 9:1 (2020). PubMed: 31908904
    • Puliyappadamba VT  et al. Opposing effect of EGFRWT on EGFRvIII-mediated NF-?B activation with RIP1 as a cell death switch. Cell Rep 4:764-75 (2013). PubMed: 23972990
    • McComb S  et al. cIAP1 and cIAP2 limit macrophage necroptosis by inhibiting Rip1 and Rip3 activation. Cell Death Differ : (2012). PubMed: 22576661
    • Huang Y  et al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell 22:1389-97 (2011). WB ; Human . PubMed: 21307340
    • Wang Z  et al. Inhibition of Hepatitis B Virus Replication by cIAP2 Involves Accelerating the Ubiquitin-Proteasome-Mediated Destruction of Polymerase. J Virol 85:11457-67 (2011). PubMed: 21865390
    • Mekhail K  et al. Identification of a common subnuclear localization signal. Mol Biol Cell 18:3966-77 (2007). WB ; Human . PubMed: 17652456

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-9 of 9 Abreviews or Q&A

    Western blot abreview for Anti-cIAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DU145 Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    DU145 Prostate cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Feb 10 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-cIAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP C42B prostate cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    LNCaP C42B prostate cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Jun 25 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-cIAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution low pH
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Prostate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formalin
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Jun 01 2018

    Western blot abreview for Anti-cIAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (T cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 10 2018

    Western blot abreview for Anti-cIAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Endothelial cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Endothelial cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. chandra mohan kurapaty venkatapoorna

    Verified customer

    Submitted Jun 12 2017

    Flow Cytometry abreview for Anti-cIAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (CLL patiant cells)
    Permeabilization
    Yes - BD kit 554714
    Gating Strategy
    live cells
    Specification
    CLL patiant cells
    Fixation
    BD kit 554714
    Read More

    Mrs. Naama Gil

    Verified customer

    Submitted Mar 30 2016

    Western blot abreview for Anti-cIAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (vaginal epithelial cells)
    Loading amount
    30 µg
    Specification
    vaginal epithelial cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2012

    Question

    Actually I tried both incubating with secondaries separately and jointly.

    There was no difference so far.

    I don't know how those antibodies would compete with each other since they are against different hosts. One is anti rabbit another one is anti mouse.

    Ive been doing ICC and IHC for quite a time and never had that problem.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 24 2012

    Answer

    Thank you for your reply.

    Many antibodies will be suitable for simultaneous incubation, however, some antibodies will interfere with each other's binding. For example, if an antibody hasa weaker binding affinity, it is often observed that incubation in a solution that contains a high concentration of other proteins can prevent effective binding. For this reason, it is often recommended that blocking agents in the antibody diluent be kept at a concentration of 1% or lower. Incubating multiple primary antibodies simultaneously may result in reduced binding efficacy.

    As I mentioned earlier, if you are unsatisfied with the results, please let me know and I will be happy to offer you a refund or credit on your original order.

    Read More

    Abcam Scientific Support

    Answered on Feb 24 2012

    Question

    Our lab has recently purchased the cIAP2 antibody (ab23423) for westerns (our westerns are on Peripheral Blood Mononucleocites extracted from Blood and Spleen). I was wondering if you have a positive control for sale or have any suggestions for a good positive control that we can use? Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on May 07 2010

    Answer

    Thank you for your enquiry. For western blot, HeLa cell lysates can be used as a positive control, as seen in our laboratory testings (datasheet WB image). Also, according to SwissProt (http://www.uniprot.org/uniprot/Q13489), the protein is highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes. Therefore, these could also be used as a positive control. I hope this information will be useful. If there is anything that I can help you with, please do not hesitate to contact me. Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on May 07 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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