Overview

  • Product name
  • Description
    Rabbit polyclonal to cIAP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Available on request. (Peptide available as ab38273.)

Properties

Applications

Our Abpromise guarantee covers the use of ab2540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with cIAP2 peptide (ab38273).

Target

  • Function
    Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
  • Tissue specificity
    Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
  • Involvement in disease
    Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 CARD domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIP 1 antibody
    • AIP1 antibody
    • API 2 antibody
    • API2 antibody
    • Apoptosis inhibitor 2 antibody
    • Baculoviral IAP repeat containing 3 antibody
    • Baculoviral IAP repeat containing protein 3 antibody
    • Baculoviral IAP repeat-containing protein 3 antibody
    • BIRC 3 antibody
    • BIRC3 antibody
    • BIRC3_HUMAN antibody
    • C IAP2 antibody
    • C-IAP2 antibody
    • CIAP 2 antibody
    • CIAP2 antibody
    • HAIP 1 antibody
    • HAIP1 antibody
    • HIAP 1 antibody
    • HIAP-1 antibody
    • HIAP1 antibody
    • IAP homolog C antibody
    • IAP-1 antibody
    • Inhibitor of apoptosis protein 1 antibody
    • MALT 2 antibody
    • MALT2 antibody
    • Mammalian IAP homolog C antibody
    • MIHC antibody
    • RING finger protein 49 antibody
    • RNF49 antibody
    • TNFR2 TRAF signaling complex protein 1 antibody
    • TNFR2 TRAF signalling complex protein antibody
    • TNFR2-TRAF-signaling complex protein 1 antibody
    see all

References

This product has been referenced in:
  • Lopergolo A  et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB ; Mouse . Read more (PubMed: 19223905) »
  • Esposito I  et al. Overexpression of cellular inhibitor of apoptosis protein 2 is an early event in the progression of pancreatic cancer. J Clin Pathol 60:885-95 (2007). Read more (PubMed: 16775116) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
25 µg
Specification
293T
Treatment
cIAP2 siRNA
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Nov 01 2005

Question

ab2540 lot # 61468 The problem I am having with ab2540 is that it does not appear to detect cIAP2 in the mouse. Using total cell lysate from MCF10A (human mammary epithelial) cells as a positive control ab2540 detects a single band at approximately 65 kDa that is likely to represent cIAP2 (see attatched Figure 1). However, no equivalent band is detected in either the FSK7 (mouse mammary epithelial) or MEF (mouse embryonic fibroblast) lanes. The left hand side of Figure 1 represents protein extracted from mouse tissue by homogenising in RIPA buffer containing protease and phosphatase inhibitors. Again, no band appears likely to represent mouse cIAP2. The expression of cIAP2 in the tissue samples used for the western blot has been confirmed by reverse transcriptase PCR. Western Blot Protocol: Ab2540 was raised in the rabbit and 'secondary only' control blots have been performed to demonstrate that any bands visible are due to the primary antibody. Approximately 20 µg of protein is loaded in each lane of an 8% SDS-PAGE gel and separated under reducing conditions. Transfer to a nitrocellulose membrane is achieved at 100 V for 90 minutes. The membrane is then blocked using 5 % non-fat milk/ 0.1% Tween in PBS either for 2 hours at room temp or overnight at 4 degrees. Primary antibody solutions of between 1 and 3 µg/ml were tested with 2 µg/ml been the optimum. The blot was incubated in primary antibody for 2 hours at room temperature. Between primary and secondary antibody the membrane is washed 4 x 5 minutes with PBS/0.1 % Tween. The secondary antibody was HRP-conjugated Donkey anti-rabbit IgG from Jackson labs, which is used at 1:10,000 and incubated with the blot for 1 hr. Before detection of bound secondary antibody the 4 x 5 minute washes are repeated and supplemented with a futher 5 minutes in PBS alone. Detection is achieved using ECL detection kit (Amersham) and Kodak Biomax XAR film. Regards Tom Owens

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Answer

Thank you for taking the time to submit your concerns and protocol, having now read through the information that you have sent me. There are a few suggestions that may be made. 1) From the gel the bands that you are observing in lanes two and three of the "mouse tissue" seem to correlate with the bands that you observe in lane 14 and 15 that you have identified as the target? Re-run the gel on a ten well gel. Run the sample with a molecular weight ladder, this is currently not present (unless the ladder has been chopped off?). Wider wells will enable you to view the gel more clearly. Run these lanes against a positive control, previous customers have detected single bands using HCT116 lysate 2) What loading control have you used to ensure that all the wells have been loaded evenly? 2) What is the tissue are you using that you refer to as "mouse tissue"? To what extent is the target present in this tissue? If you are still experiencing problems than please get back in touch with us. Good luck with your research.

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Answer

Thank you for your email. This antibody has been tested for cross-reactivity with both human and mouse, however we do not have any images available. If you have any more questions, please contact us again.

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Question
Answer

We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. This product falls into the latter category. The identity of our suppliers remains commercially sensitive information.

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Answer

The immunogen is 507-524 of human cIAP-2. The antibody has not been tested with rat sample, and has only been tested for application in Western blotting. Please refer to the online datasheet for ab2540 for any references that we have.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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