Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E40] to cIAP2
- Suitable for: Flow Cyt, WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-cIAP2 antibody [E40]
See all cIAP2 primary antibodies
DescriptionRabbit monoclonal [E40] to cIAP2
SpecificityThis antibody is specific for human cIAP2. It does not cross-react with any other IAP family members.
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IPmore details
Unsuitable for: ICC
Species reactivityReacts with: Human
Synthetic peptide within Human cIAP2 aa 400-500. The exact sequence is proprietary.
- WB: Daudi cell lysate IHC-P: Human spleen tissue, Human breast carcinomaIP: Daudi cell lysate Flow Cyt: Raji cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab32059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000. Predicted molecular weight: 72 kDa.
For unpurified use at 1/1000.
|IHC-P||1/600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||Use a concentration of 30 mg/ml.
For unpurified use at 5 µg/mL.
FunctionApoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
Tissue specificityHighly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
Involvement in diseaseNote=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
Sequence similaritiesBelongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 CARD domain.
Contains 1 RING-type zinc finger.
- Information by UniProt
- AIP 1 antibody
- AIP1 antibody
- API 2 antibody
Anti-cIAP2 antibody [E40] (ab32059) at 1/50000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution (unpurified) + Daudi cell lysate.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling cIAP2 with purified ab32059 at 1/600 dilution (1.12 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling cIAP2 with purified ab32059 at 1/70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
ab32059 (purified) at 1/30 dilution (2ug) immunoprecipitating cIAP2 in Daudi whole cell lysates.
Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates 10ug
Lane 2 (+): ab32059 & Daudi whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32059 in Daudi whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
ab32059 (unpurified) at a 1/50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059 (unpurified).
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 72kDa; cIAP2
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32059 has been referenced in 37 publications.
- Patel S et al. RIP1 inhibition blocks inflammatory diseases but not tumor growth or metastases. Cell Death Differ 27:161-175 (2020). PubMed: 31101885
- Chen M et al. Smac mimetic promotes TNF-a to induce apoptosis of gallbladder carcinoma cells. Cell Signal 72:109654 (2020). PubMed: 32334028
- Ma H et al. Identification of reciprocal microRNA-mRNA pairs associated with metastatic potential disparities in human prostate cancer cells and signaling pathway analysis. J Cell Biochem 120:17779-17790 (2019). PubMed: 31127646
- Lukosiute-Urboniene A et al. Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer. World J Gastroenterol 25:205-219 (2019). PubMed: 30670910
- Ning Y et al. Changes in the NF-?B signaling pathway in juvenile and adult patients with Kashin-Beck disease. Exp Cell Res 379:140-149 (2019). PubMed: 30951708