Overview

  • Product name
    Anti-cIAP2 antibody [E40]
    See all cIAP2 primary antibodies
  • Description
    Rabbit monoclonal [E40] to cIAP2
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for human cIAP2. It does not cross-react with any other IAP family members.
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human cIAP2 aa 400-500. The exact sequence is proprietary.

  • Positive control
    • Daudi cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/100.
IP Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Apoptotic suppressor. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form an heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2).
    • Tissue specificity
      Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
    • Involvement in disease
      Note=A chromosomal aberration involving BIRC3 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with MALT1. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.
    • Sequence similarities
      Belongs to the IAP family.
      Contains 3 BIR repeats.
      Contains 1 CARD domain.
      Contains 1 RING-type zinc finger.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIP 1 antibody
      • AIP1 antibody
      • API 2 antibody
      • API2 antibody
      • Apoptosis inhibitor 2 antibody
      • Baculoviral IAP repeat containing 3 antibody
      • Baculoviral IAP repeat containing protein 3 antibody
      • Baculoviral IAP repeat-containing protein 3 antibody
      • BIRC 3 antibody
      • BIRC3 antibody
      • BIRC3_HUMAN antibody
      • C IAP2 antibody
      • C-IAP2 antibody
      • CIAP 2 antibody
      • CIAP2 antibody
      • HAIP 1 antibody
      • HAIP1 antibody
      • HIAP 1 antibody
      • HIAP-1 antibody
      • HIAP1 antibody
      • IAP homolog C antibody
      • IAP-1 antibody
      • Inhibitor of apoptosis protein 1 antibody
      • MALT 2 antibody
      • MALT2 antibody
      • Mammalian IAP homolog C antibody
      • MIHC antibody
      • RING finger protein 49 antibody
      • RNF49 antibody
      • TNFR2 TRAF signaling complex protein 1 antibody
      • TNFR2 TRAF signalling complex protein antibody
      • TNFR2-TRAF-signaling complex protein 1 antibody
      see all

    Images

    • Anti-cIAP2 antibody [E40] (ab32059) at 1/1000 dilution + Daudi cell lysate.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa

    • ab32059 at a 1:50 dilution staining cIAP2 in human normal spleen, using Immunohistochemistry, Paraffin Embedded Tissue.
    • cIAP2 was immunoprecipitated using 0.5mg Daudi whole cell extract, 5µg of Rabbit polyclonal to cIAP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Daudi whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32059.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 72kDa; cIAP2

    References

    This product has been referenced in:
    • Lukosiute-Urboniene A  et al. Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer. World J Gastroenterol 25:205-219 (2019). Read more (PubMed: 30670910) »
    • Wang L  et al. CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC. Oncol Rep 39:951-966 (2018). Read more (PubMed: 29286141) »
    See all 28 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-2 of 2 Abreviews

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (Epithelial)
    Specification
    Epithelial
    Treatment
    50 ng/TNF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 27 2014

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% acrylamyde)
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up