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Hello, In my first email I mentioned that left picture with high background is the membrane incubated with monoclonal antibody. The right picture represents the polyclonal antibody. I did increase the contrast so the band would become visible.that is the reason having high background. Thank you
Asked on Dec 20 2011
Thanks for your reply and for your patience while i have been in touch with the lab. I have heard back from the lab, and I don't see anything unusual in their protocol so I don't have a great explanation for these results. At this point, I would suggest trying a different BIRC3 antibody (though I know you've already tried 2). I've looked through the antibodies that we have to this target, and I think that ab23423 looks promising. The WB image on the datasheet shows some extra bands (thought to be cleavage fragments or non-specific bands), but we only have 1 complaint on this antibody since it has been sold. Ab23423 has also been used in 3 references. As I mentioned before, these antibodies are covered by our guarantee, so I can issued a credit or refund if you would prefer. I apologize for all of the difficulty with these antibodies, and I'm hopeful that we can identify one that will work for you. I look forward to hearing from you. Please let me know how you would like to proceed.
Answered on Dec 20 2011