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My doubt is related with the antigen retrieval step used to obtain these specific results. Do you recommend an antigen retrieval step with these antibodies?.
Asked on Jan 29 2014
Please notice, these protocols may be optimised, and could not work with your specific tissue.
Ab32059: Steam slides in 0.01M Sodium Citrate Buffer, pH 6.0 at 125°C for 30 seconds followed by 10 seconds at 90° C into a decloaking chamber / pressure cooker. Removes slides and rinse in TBST before proceeding to the staining step.
Ab113237: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) – 1 minute at RT.
Ariana VeigaAbcam Scientific Support
Answered on Jan 29 2014