Western blot abreview for Anti-cIAP2 antibody [E40]

Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% acrylamyde)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-0,05%Tween -5%Milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit DAKO
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500

Detection

Detection method
Super Signal WestDura
Bands
Specific: 70 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
TNFalpha 250u/ml 8hrs

Abcam user community

Verified customer

Submitted Nov 04 2013

Sign up