Western blot abreview for Anti-cIAP2 antibody [E40]

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Epithelial)
Specification
Epithelial
Treatment
50 ng/TNF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: ECL Anti-Rabbite
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL prime
Bands
Specific: 72 kDa Non-specific: 100, 80, 70 kDa
Exposure
3 minute(s) and 0 second(s)
Negative control
Neisseria gonorrhoeae lysate (GC WCL)
Positive control
HeLa whole cell lysate (WCL) and TNF treated

Additional data

Additional Notes
The antibody binds nicely to cIAP2 unfortunately it also nicely binds to niesseria gonorrhoeae at 80kd and 70kd, making it difficult to distinguish from the human cIAP2. Thus, I cannot tell how the bacteria modulates cIAP2 because the antibody binds to the pathogen. GC WCL: Nesseria gonorrhoeae whole bug lysate HeLa WCL: whole cell lysate Un: Unstimulated cells MOI: multiplicity of infection

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Submitted Feb 27 2014

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