The antibody binds nicely to cIAP2 unfortunately it also nicely binds to niesseria gonorrhoeae at 80kd and 70kd, making it difficult to distinguish from the human cIAP2. Thus, I cannot tell how the bacteria modulates cIAP2 because the antibody binds to the pathogen.
GC WCL: Nesseria gonorrhoeae whole bug lysate
HeLa WCL: whole cell lysate
Un: Unstimulated cells
MOI: multiplicity of infection
Abcam user community
Submitted Feb 27 2014
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