Anti-CIAPIN1 antibody (ab154904)
Key features and details
- Rabbit polyclonal to CIAPIN1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CIAPIN1 antibody
See all CIAPIN1 primary antibodies -
Description
Rabbit polyclonal to CIAPIN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-312 of Human CIAPIN1 (Uniprot ID: Q6FI81).
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Positive control
- Jurkat, Raji, K562, THP1, HL60, NCI-H929, NIH 3T3, JC and BCL1 whole cell lysates; Human SkHep1 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 34 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
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Function
May be required for the maturation of extramitochondrial Fe/S proteins (By similarity). Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells. -
Tissue specificity
Ubiquitously expressed. Highly expressed in heart, liver and pancreas. -
Sequence similarities
Belongs to the anamorsin family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57019 Human
- Entrez Gene: 109006 Mouse
- Omim: 608943 Human
- SwissProt: Q6FI81 Human
- SwissProt: Q8WTY4 Mouse
- Unigene: 4900 Human
- Unigene: 27582 Mouse
- Unigene: 472546 Mouse
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Alternative names
- 2810413N20Rik antibody
- Anamorsin antibody
- CIAPIN 1 antibody
see all
Images
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Western blot - Anti-CIAPIN1 antibody (ab154904)All lanes : Anti-CIAPIN1 antibody (ab154904) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : THP1 whole cell lysate
Lane 5 : HL60 whole cell lysate
Lane 6 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
10% SDS PAGE -
Western blot - Anti-CIAPIN1 antibody (ab154904)All lanes : Anti-CIAPIN1 antibody (ab154904) at 1/3000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIAPIN1 antibody (ab154904)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SkHep1 xenograft tissue labeling CIAPIN1 with ab154904 at 1/500 dilution.
Protocols
References (3)
ab154904 has been referenced in 3 publications.
- Zheng J et al. MiRNA-195-5p Functions as a Tumor Suppressor and a Predictive of Poor Prognosis in Non-small Cell Lung Cancer by Directly Targeting CIAPIN1. Pathol Oncol Res N/A:N/A (2019). PubMed: 30637589
- Chen X et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). PubMed: 28858244
- Wang JH et al. Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncol Lett 13:4695-4700 (2017). WB . PubMed: 28588724
