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    cib1kip-antibody-ab220606.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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Anti-CIB1/KIP antibody (ab220606)

  • Datasheet
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Western blot - Anti-CIB1/KIP antibody (ab220606)
  • Immunocytochemistry/ Immunofluorescence - Anti-CIB1/KIP antibody (ab220606)

Key features and details

  • Rabbit polyclonal to CIB1/KIP
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CIB1/KIP antibody
    See all CIB1/KIP primary antibodies
  • Description

    Rabbit polyclonal to CIB1/KIP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow
  • Immunogen

    Recombinant fragment corresponding to Human CIB1/KIP aa 105-187.
    Sequence:

    DIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLID NILEESDIDRDGTINLSEFQHVISRSPDFASSF


    Database link: Q99828
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: RT-4 cell lysate. ICC/IF: A549 cells.
  • General notes

     This product was previously labelled as CIB1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CIB1/KIP protein (ab78759)

Applications

Our Abpromise guarantee covers the use of ab220606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 22 kDa.

Target

  • Function

    May convert the inactive conformation of integrin alpha-IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 2 EF-hand domains.
  • Cellular localization

    Membrane. Cytoplasm. Nucleus. Cell projection > filopodium. Apical cell membrane. Localizes to the perinuclear region in the presence of NBR1. Colocalizes with TAS1R2 in apical regions of taste receptor cells.
  • Target information above from: UniProt accession Q99828 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10519 Human
    • Entrez Gene: 23991 Mouse
    • Entrez Gene: 81823 Rat
    • Omim: 602293 Human
    • SwissProt: Q99828 Human
    • SwissProt: Q9Z0F4 Mouse
    • SwissProt: Q9R010 Rat
    • Unigene: 715556 Human
    • Unigene: 30217 Mouse
    • Unigene: 833 Rat
    see all
  • Alternative names

    • Calcium and integrin binding 1 (calmyrin) antibody
    • Calcium and integrin binding protein 1 antibody
    • Calcium and integrin-binding protein 1 antibody
    • Calcium- and integrin-binding protein antibody
    • Calmyrin antibody
    • CIB antibody
    • CIB1 antibody
    • CIB1_HUMAN antibody
    • CIBP antibody
    • DNA PKcs interacting protein antibody
    • DNA-dependent protein kinase interacting protein antibody
    • DNA-PK interaction protein antibody
    • DNA-PKcs-interacting protein antibody
    • Human Snk interacting protein 2 to 28 mRNA complete cds antibody
    • Kinase interacting protein antibody
    • Kinase-interacting protein 1 antibody
    • Kinase-interacting protein antibody
    • KIP antibody
    • KIP1 antibody
    • PRKDCIP antibody
    • SIP2 28 antibody
    • SIP2-28 antibody
    • SNK interacting protein 2 to 28 antibody
    • SNK-interacting protein 2-28 antibody
    see all

Images

  • Western blot - Anti-CIB1/KIP antibody (ab220606)
    Western blot - Anti-CIB1/KIP antibody (ab220606)
    Anti-CIB1/KIP antibody (ab220606) at 1/2500 dilution + RT-4 cell lysate

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CIB1/KIP antibody (ab220606)
    Immunocytochemistry/ Immunofluorescence - Anti-CIB1/KIP antibody (ab220606)

    Immunofluorescent analysis of PFA fixed, triton X-100 permeabilized A549 cells labeling CIB1/KIP with ab220606 at 4 µg/mL (green).

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab220606? Please let us know so that we can cite the reference in this datasheet.

    ab220606 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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