Overview

  • Product name

    CIC-C3d ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 16 6.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 13.9%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CIC-C3d in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of CIC-C3d in Human serum and plasma.

    A 96-well plate has been precoated with anti-C3d antibodies. Standards, controls and test samples are added to the wells and incubated. Immune complexes (CIC) in the samples and standards bind to the precoated antibody. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized CIC-C3d. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of CIC-C3d in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 2 x 50ml
    Anti-CIC-C3d Coated Microplate (12 x 8 wells) 1 unit
    CIC-C3d Anti-IgG HRP Conjugate 1 x 0.5ml
    CIC-C3d Negative Control 1 x 1.5ml
    CIC-C3d Positive Control 1 x 1.5ml
    CIC-C3d Standard 0 - 0 µg Eq/mL 1 x 1.5ml
    CIC-C3d Standard 1 - 16 µg Eq/mL 1 x 1.5ml
    CIC-C3d Standard 2 - 64 µg Eq/mL 1 x 1.5ml
    Conjugate Buffer 1 x 20ml
    Cover Foil 1 unit
    Incubation Buffer 1 x 50ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab178665.

Protocols

References

ab178665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer


I apologize for the confusion with this protocol.

Here are the concentrations:

Standard 0 0 µg Eq/ml

Standard 1 16 µg Eq/ml

Standard 2 64 µg Eq/ml

Read More

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