Key features and details
- Rabbit polyclonal to CID
- Suitable for: WB, IHC-FoFr, ICC, ICC/IF
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Product nameAnti-CID antibody
DescriptionRabbit polyclonal to CID
Tested applicationsSuitable for: WB, IHC-FoFr, ICC, ICC/IFmore details
Species reactivityReacts with: Drosophila melanogaster
Synthetic peptide corresponding to Drosophila melanogaster CID aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Drosophila Embryo Lysate;
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab10887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution (PMID 19137063).
WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHistone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
Sequence similaritiesBelongs to the histone H3 family.
Cellular localizationNucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha.
- Information by UniProt
- CENP A antibody
- CENP-A homolog antibody
- CENPA / CID antibody
Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg
Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
ab10887 has been referenced in 10 publications.
- Huang A et al. Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading. Nucleic Acids Res 47:10754-10770 (2019). PubMed: 31535131
- Strunov A et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol 16:68 (2018). PubMed: 29907103
- Moshkin YM et al. Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A. PLoS Genet 9:e1003719 (2013). PubMed: 24086141
- Yan R et al. SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster. J Cell Biol 188:335-49 (2010). ICC/IF ; Drosophila melanogaster . PubMed: 20142422
- Podhraski V et al. CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila. PLoS One 5:e10120 (2010). WB, ICC/IF ; Drosophila melanogaster . PubMed: 20396651
- Dubruille R et al. Specialization of a Drosophila capping protein essential for the protection of sperm telomeres. Curr Biol 20:2090-9 (2010). IHC ; Drosophila melanogaster . PubMed: 21093267
- Löhler J et al. Immunohistochemical characterisation of cell-type specific expression of CK1delta in various tissues of young adult BALB/c mice. PLoS ONE 4:e4174 (2009). IHC-FoFr . PubMed: 19137063
- Kotadia S et al. PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen. Oncogene 27:6334-46 (2008). ICC/IF . PubMed: 18663356
- Khetani RS & Bickel SE Regulation of meiotic cohesion and chromosome core morphogenesis during pachytene in Drosophila oocytes. J Cell Sci 120:3123-37 (2007). ICC/IF ; Drosophila melanogaster . PubMed: 17698920
- Cesario JM et al. Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators. J Cell Sci 119:4770-80 (2006). PubMed: 17077127