• Product name
    Anti-CID antibody
  • Description
    Rabbit polyclonal to CID
  • Host species
  • Tested applications
    Suitable for: WB, IHC-FoFr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide derived from residues 1 - 100 of Drosophila melanogaster CID.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • Drosophila Embryo Lysate;


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab10887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Suggested working dilution is 1/100 (see PMID: 17077127).
    IHC-FoFr: Use at an assay dependent dilution (PMID 19137063).
    WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
    • Sequence similarities
      Belongs to the histone H3 family.
    • Cellular localization
      Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha.
    • Information by UniProt
    • Database links
      • Alternative names
        • CENP A antibody
        • CENP-A homolog antibody
        • CENPA / CID antibody
        • CENPA antibody
        • Centromere identifier antibody
        • Centromere identifier protein antibody
        • Centromere protein A antibody
        • cid antibody
        • CID/CENP A antibody
        • CID_DROME antibody
        • Histone H3 like centromeric protein cid antibody
        • Histone H3-like centromeric protein cid antibody
        see all


      • Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
      • Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg

        Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 26 kDa
        Observed band size: 26 kDa
        Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)


      This product has been referenced in:
      • Strunov A  et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol 16:68 (2018). Read more (PubMed: 29907103) »
      • Moshkin YM  et al. Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A. PLoS Genet 9:e1003719 (2013). Read more (PubMed: 24086141) »
      See all 9 Publications for this product

      Customer reviews and Q&As

      1-6 of 6 Abreviews or Q&A

      Abcam has not validated the combination of species/application used in this Abreview.
      IHC - Wholemount
      Fruit fly (Drosophila melanogaster) Embryo (Drosophila embryo)
      Drosophila embryo

      Jung Wan Mok

      Verified customer

      Submitted Jun 06 2016


      Thank you for your reply. Unfortunately, since the new lot is still in production, I am unable to set up an automatic alert for when it comes into stock or an order for a lot that doesn't exist yet. I have issued you a refund (Credit Note ***) and will check periodically for the new lot of ab10887. Once the new batch is available, you can place the order like normal and specify the lot number I will send you. The charge for the antibody and shipping / packing will be refunded automatically to the card used to make the purchase. If you have any questions about this refund, please contact us.credits@abcam.com.

      Read More


      Thank you for your patience. I checked with my colleagues in our production lab and we are currently scheduled to begin immunizations for a new batch of ab10887 in one week. This would mean that a new lot would be available for sale in a couple of months. Unfortunately, I am unable to give a more accurate timeframe because it will depend on how the antibody performs in QC.

      We do currently have additional stock of the same batch of ab10887 and I would be happy to send you a new vial if you would like to try one. Alternatively, I could offer you a replacement with an antibody to a different target, or a credit or refund. Please let me know how you would like to proceed and I will be happy to assist you further.

      Read More


      According to our records, ab10887 was showing lot-to-lot variation and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting some images to forward to the lab, and we would be happy to send a new lot if we have one in stock. If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records. We wish you the best of luck with your research and look forward to hearing from you. Please let us know if there is anything else that we can do for you.

      Read More
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Fruit fly (Drosophila melanogaster) Cell (DM2 cells)
      DM2 cells
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 1

      Dr. Beth Sullivan

      Verified customer

      Submitted Oct 05 2005


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