Overview

  • Product name
    Anti-CID antibody
  • Description
    Rabbit polyclonal to CID
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-FoFr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide derived from residues 1 - 100 of Drosophila melanogaster CID.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • Drosophila Embryo Lysate;

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-FoFr
ICC
ICC/IF
  • Application notes
    ICC/IF: Suggested working dilution is 1/100 (see PMID: 17077127).
    IHC-FoFr: Use at an assay dependent dilution (PMID 19137063).
    WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
    • Sequence similarities
      Belongs to the histone H3 family.
    • Cellular localization
      Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha.
    • Information by UniProt
    • Database links
      • Alternative names
        • CENP A antibody
        • CENP-A homolog antibody
        • CENPA / CID antibody
        • CENPA antibody
        • Centromere identifier antibody
        • Centromere identifier protein antibody
        • Centromere protein A antibody
        • cid antibody
        • CID/CENP A antibody
        • CID_DROME antibody
        • Histone H3 like centromeric protein cid antibody
        • Histone H3-like centromeric protein cid antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (ab10887)
        Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (ab10887)This image is courtesy of Elaine Dunleavy
        Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
      • Western blot - Anti-CID antibody (ab10887)
        Western blot - Anti-CID antibody (ab10887)
        Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg

        Secondary
        Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 26 kDa
        Observed band size: 26 kDa
        Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)

      References

      This product has been referenced in:
      • Moshkin YM  et al. Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A. PLoS Genet 9:e1003719 (2013). Read more (PubMed: 24086141) »
      • Yan R  et al. SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster. J Cell Biol 188:335-49 (2010). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 20142422) »

      See all 8 Publications for this product

      Publishing research using ab10887? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      6 reviews or Q&A

      10
      Votes: Highest First

      IHC - Wholemount abreview for Anti-CID antibody

       Poor
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      IHC - Wholemount
      Sample
      Fruit fly (Drosophila melanogaster) Embryo (Drosophila embryo)
      Specification
      Drosophila embryo
      Read More
      abreview image
      Username

      Jung Wan Mok

      Verified customer

      Submitted Jun 06 2016

      We unfortunately were not able to obtain a better result by changing our
      fixation protocol. Therefore, we ask that you issue us a credit against
      our purchase of a replacement vial of ab10887 from the new batch that has
      just gone into pro...

      Read More

      Thank you for your reply. Unfortunately, since the new lot is still in production, I am unable to set up an automatic alert for when it comes into stock or an order for a lot that doesn't exist yet. I have issued you a refund (Credit Note ***) and will...

      Read More

      Thank you for looking into this. Given the time frame involved, it
      doesn't seem like we will be able to defer our work until the next batch
      of ab10887 becomes available.
      Before we request a refund, however, we would like to see ...

      Read More

      Thank you for your reply. That is no problem, just let me know when you decide whether you would like the refund or not. If you have any further questions I would be happy to help!

      ab10887 worked previously in ICC on drosophila S2 cells, but the new vial is giving high background.

      Thank you for your patience. I checked with my colleagues in our production lab and we are currently scheduled to begin immunizations for a new batch of ab10887 in one week. This would mean that a new lot would be available for sale in a couple of mont...

      Read More

      Old lot did work in ICC, new lot does not.

      According to our records, ab10887 was showing lot-to-lot variation and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting some images to forward to the lab, and we would be happ...

      Read More

      Immunocytochemistry abreview for Anti-CID antibody

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Immunocytochemistry
      Sample
      Fruit fly (Drosophila melanogaster) Cell (DM2 cells)
      Specification
      DM2 cells
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 1
      Read More
      abreview image
      Username

      Dr. Beth Sullivan

      Verified customer

      Submitted Oct 05 2005

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up