Anti-CID antibody (ab10887)

Reviews (2)Q&A (4)References (10)

Key features and details

  • Rabbit polyclonal to CID
  • Suitable for: WB, IHC-FoFr, ICC, ICC/IF
  • Reacts with: Drosophila melanogaster
  • Isotype: IgG

Overview

  • Product name

    Anti-CID antibody

  • Description

    Rabbit polyclonal to CID

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-FoFr, ICC, ICC/IFmore details

  • Species reactivity

    Reacts with: Drosophila melanogaster

  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster CID aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16766)

  • Positive control

    • Drosophila Embryo Lysate;

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-FoFr
ICC
ICC/IF
  • Application notes
    ICC/IF: Suggested working dilution is 1/100 (see PMID: 17077127).
    IHC-FoFr: Use at an assay dependent dilution (PMID 19137063).
    WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.

    • Sequence similarities

      Belongs to the histone H3 family.

    • Cellular localization

      Nucleus. Chromosome, centromere, kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Co-expressed with yemalpha.
    • Information by UniProt

    • Database links

      • Alternative names

        • CENP A antibody
        • CENP-A homolog antibody
        • CENPA / CID antibody
        • CENPA antibody
        • Centromere identifier antibody
        • Centromere identifier protein antibody
        • Centromere protein A antibody
        • cid antibody
        • CID/CENP A antibody
        • CID_DROME antibody
        • Histone H3 like centromeric protein cid antibody
        • Histone H3-like centromeric protein cid antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (ab10887)
        Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (ab10887)This image is courtesy of Elaine Dunleavy
        Drosophila S2 cells treated with ab10887 at 1:100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887
      • Western blot - Anti-CID antibody (ab10887)
        Western blot - Anti-CID antibody (ab10887)
        Anti-CID antibody (ab10887) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg

        Secondary
        Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

        Performed under reducing conditions.

        Predicted band size: 26 kDa
        Observed band size: 26 kDa
        Additional bands at: 12 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)

      References (10)

      Publishing research using ab10887? Please let us know so that we can cite the reference in this datasheet.

      ab10887 has been referenced in 10 publications.

      • Huang A  et al. Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading. Nucleic Acids Res 47:10754-10770 (2019). PubMed: 31535131
      • Strunov A  et al. Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors. BMC Biol 16:68 (2018). PubMed: 29907103
      • Moshkin YM  et al. Histone Chaperone NAP1 Mediates Sister Chromatid Resolution by Counteracting Protein Phosphatase 2A. PLoS Genet 9:e1003719 (2013). PubMed: 24086141
      • Yan R  et al. SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster. J Cell Biol 188:335-49 (2010). ICC/IF ; Drosophila melanogaster . PubMed: 20142422
      • Podhraski V  et al. CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila. PLoS One 5:e10120 (2010). WB, ICC/IF ; Drosophila melanogaster . PubMed: 20396651
      • Dubruille R  et al. Specialization of a Drosophila capping protein essential for the protection of sperm telomeres. Curr Biol 20:2090-9 (2010). IHC ; Drosophila melanogaster . PubMed: 21093267
      • Löhler J  et al. Immunohistochemical characterisation of cell-type specific expression of CK1delta in various tissues of young adult BALB/c mice. PLoS ONE 4:e4174 (2009). IHC-FoFr . PubMed: 19137063
      • Kotadia S  et al. PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen. Oncogene 27:6334-46 (2008). ICC/IF . PubMed: 18663356
      • Khetani RS & Bickel SE Regulation of meiotic cohesion and chromosome core morphogenesis during pachytene in Drosophila oocytes. J Cell Sci 120:3123-37 (2007). ICC/IF ; Drosophila melanogaster . PubMed: 17698920
      • Cesario JM  et al. Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators. J Cell Sci 119:4770-80 (2006). PubMed: 17077127

      Customer reviews and Q&As

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      1-6 of 6 Abreviews or Q&A

      IHC - Wholemount abreview for Anti-CID antibody

       Poor
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Fruit fly (Drosophila melanogaster) Embryo (Drosophila embryo)
      Specification
      Drosophila embryo
      Read More

      Jung Wan Mok

      Verified customer

      Submitted Jun 06 2016

      Question

      We unfortunately were not able to obtain a better result by changing our
      fixation protocol. Therefore, we ask that you issue us a credit against
      our purchase of a replacement vial of ab10887 from the new batch that has
      just gone into production. We understand that the new batch is still
      several weeks away from being ready for sale.
      Can you enter an order for us now, to be shipped when the new batch of
      ab10887 actually is ready to go on sale? If not, please let me know what
      is the best way for us to obtain a vial from the new batch.

      Read More
      Answer

      Thank you for your reply. Unfortunately, since the new lot is still in production, I am unable to set up an automatic alert for when it comes into stock or an order for a lot that doesn't exist yet. I have issued you a refund (Credit Note ***) and will check periodically for the new lot of ab10887. Once the new batch is available, you can place the order like normal and specify the lot number I will send you. The charge for the antibody and shipping / packing will be refunded automatically to the card used to make the purchase. If you have any questions about this refund, please contact us.credits@abcam.com.

      Read More

      Question

      Thank you for looking into this. Given the time frame involved, it
      doesn&apos;t seem like we will be able to defer our work until the next batch
      of ab10887 becomes available.
      Before we request a refund, however, we would like to see whether the
      antibody might perform any better for us if we use a methanol fixation
      protocol. We can do the experiment either this Friday or next Monday, and
      if we continue to get high background we would then get back to you right
      away in order to seek a refund.
      So, I guess I need to know whether you are able to wait a few more days
      for us to make a decision?

      Read More
      Answer

      Thank you for your reply. That is no problem, just let me know when you decide whether you would like the refund or not. If you have any further questions I would be happy to help!

      Read More

      Question

      ab10887 worked previously in ICC on drosophila S2 cells, but the new vial is giving high background.

      Read More
      Answer

      Thank you for your patience. I checked with my colleagues in our production lab and we are currently scheduled to begin immunizations for a new batch of ab10887 in one week. This would mean that a new lot would be available for sale in a couple of months. Unfortunately, I am unable to give a more accurate timeframe because it will depend on how the antibody performs in QC.

      We do currently have additional stock of the same batch of ab10887 and I would be happy to send you a new vial if you would like to try one. Alternatively, I could offer you a replacement with an antibody to a different target, or a credit or refund. Please let me know how you would like to proceed and I will be happy to assist you further.

      Read More

      Question

      Old lot did work in ICC, new lot does not.

      Read More
      Answer

      According to our records, ab10887 was showing lot-to-lot variation and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting some images to forward to the lab, and we would be happy to send a new lot if we have one in stock. If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records. We wish you the best of luck with your research and look forward to hearing from you. Please let us know if there is anything else that we can do for you.

      Read More

      Immunocytochemistry abreview for Anti-CID antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Fruit fly (Drosophila melanogaster) Cell (DM2 cells)
      Specification
      DM2 cells
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 1
      Read More

      Dr. Beth Sullivan

      Verified customer

      Submitted Oct 05 2005

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