Overview

  • Product name

  • Description

    Rabbit polyclonal to CIDE A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-190 of Human CIDE A

  • Positive control

    • 293T, A431 and HeLa cell lines; HepG2 whole cell lysate (ab7900); HeLa cells; MCF-7 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICARD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B (for cell death inducing DFF like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE A is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE A is expressed in many tissues.
  • Cellular localization

    Nuclear. Cytoplasm; mitochondrial envelope; lipid droplet.
  • Database links

  • Alternative names

    • Cell death activator CIDE A antibody
    • Cell Death Inducing DFFA Like Effector A antibody
    • cell death inducing DNA fragmentation factor, alpha subunit like effector A antibody
    • CIDE-A antibody
    • CIDEA antibody
    see all

Images

  • Anti-CIDE A antibody (ab151577) at 1/5000 dilution + HepG2 cell lysate at 30 µg

    Predicted band size: 25 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded MCF-7 xenograft labeling CIDE A with ab151577 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa, labeling CIDE A with ab151577 1/200 dilution.

References

This product has been referenced in:

  • Zhang N  et al. Goserelin promotes the apoptosis of epithelial ovarian cancer cells by upregulating forkhead box O1 through the PI3K/AKT signaling pathway. Oncol Rep 39:1034-1042 (2018). Read more (PubMed: 29286125) »
See 1 Publication for this product

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