Key features and details
- Rabbit polyclonal to CIDE A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CIDE A antibody
See all CIDE A primary antibodies
DescriptionRabbit polyclonal to CIDE A
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to a region within amino acids 1-190 of Human CIDE A
- 293T, A431 and HeLa cell lines; HepG2 whole cell lysate (ab7900); HeLa cells; MCF-7 xenograft tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
RelevanceApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICARD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B (for cell death inducing DFF like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE A is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE A is expressed in many tissues.
Cellular localizationNuclear. Cytoplasm; mitochondrial envelope; lipid droplet.
- Cell death activator CIDE A antibody
- Cell Death Inducing DFFA Like Effector A antibody
- cell death inducing DNA fragmentation factor, alpha subunit like effector A antibody
Anti-CIDE A antibody (ab151577) at 1/5000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE
Immunohistochemical analysis of paraffin-embedded MCF-7 xenograft labeling CIDE A with ab151577 at 1/500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, labeling CIDE A with ab151577 1/200 dilution.
ab151577 has been referenced in 1 publication.
- Zhang N et al. Goserelin promotes the apoptosis of epithelial ovarian cancer cells by upregulating forkhead box O1 through the PI3K/AKT signaling pathway. Oncol Rep 39:1034-1042 (2018). PubMed: 29286125