Anti-CIDE A antibody (ab191193)
Key features and details
- Rabbit polyclonal to CIDE A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CIDE A antibody
See all CIDE A primary antibodies -
Description
Rabbit polyclonal to CIDE A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CIDE A aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: O60543 -
Positive control
- Human liver tissue; CEM cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100. |
Target
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Function
Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. -
Tissue specificity
Expressed in omental and subcutaneous adipose tissue (at protein level). -
Involvement in disease
In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle. -
Sequence similarities
Contains 1 CIDE-N domain. -
Cellular localization
Lipid droplet. Nucleus. Enriched at lipid droplet contact sites. Has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed (PubMed:18509062). - Information by UniProt
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Database links
- Entrez Gene: 1149 Human
- Omim: 604440 Human
- SwissProt: O60543 Human
- Unigene: 249129 Human
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Alternative names
- Cell death activator CIDE A antibody
- Cell death activator CIDE-A antibody
- Cell Death Inducing DFFA Like Effector A antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab191193 has been referenced in 1 publication.
- Dong Y et al. Transcriptome profiles of fatty acid metabolism-related genes and immune infiltrates identify hot tumors for immunotherapy in cutaneous melanoma. Front Genet 13:860067 (2022). PubMed: 36199579