Key features and details
- Rabbit polyclonal to CIDE A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CIDE A antibody
See all CIDE A primary antibodies
DescriptionRabbit polyclonal to CIDE A
SpecificityNo cross activity to CIDE B
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Brain (Human) Tissue Lysate - adult normal tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 20 µg/ml.|
RelevanceApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICARD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B (for cell death inducing DFF like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE A is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE A is expressed in many tissues.
Cellular localizationNuclear. Cytoplasm; mitochondrial envelope; lipid droplet.
- Cell death activator CIDE A antibody
- Cell Death Inducing DFFA Like Effector A antibody
- cell death inducing DNA fragmentation factor, alpha subunit like effector A antibody
All lanes : Anti-CIDE A antibody (ab62343) at 1/2000 dilution
Lane 1 : Human brain tissue lysate in the absence of the immunizing peptide
Lane 2 : Human brain tissue lysate in the presence of the immunizing peptide
Predicted band size: 25 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
ab62343 at 5ug/ml staining human brain tissue by IHC-P.
Immunofluorescence of CIDE-A in Human Brain cells using ab62343 at 20 ug/ml.
ab62343 has been referenced in 1 publication.
- Sun X et al. High expression of cell death-inducing DFFA-like effector a (CIDEA) promotes milk fat content in dairy cows with clinical ketosis. J Dairy Sci N/A:N/A (2018). PubMed: 30594378