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    cide-a-antibody-ab8402.pdf

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Cell Biology Apoptosis Nucleus DNAses
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Anti-CIDE A antibody (ab8402)

  • Datasheet
  • SDS
Submit a review Submit a question References (9)

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Western blot - Anti-CIDE A antibody (ab8402)
  • Immunocytochemistry/ Immunofluorescence - Anti-CIDE A antibody (ab8402)

Key features and details

  • Rabbit polyclonal to CIDE A
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG

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Overview

  • Product name

    Anti-CIDE A antibody
    See all CIDE A primary antibodies
  • Description

    Rabbit polyclonal to CIDE A
  • Host species

    Rabbit
  • Specificity

    Does not recognise CIDE-B.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse CIDE A. The antibody was raised against an 18 amino acid peptide within the last 50 amino acids of mouse CIDE A.
    Database link: O70302
    (Peptide available as ab8434)

  • Positive control

    • WB: Mouse heart tissue lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Light chain type

    unknown
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • DNAses
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • DNAses
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500. Predicted molecular weight: 25 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
Notes
WB
1/500. Predicted molecular weight: 25 kDa.
ICC/IF
Use a concentration of 20 µg/ml.

Target

  • Function

    Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.
  • Tissue specificity

    Expressed in omental and subcutaneous adipose tissue (at protein level).
  • Involvement in disease

    In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle.
  • Sequence similarities

    Contains 1 CIDE-N domain.
  • Cellular localization

    Lipid droplet. Nucleus. Enriched at lipid droplet contact sites. Has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed (PubMed:18509062).
  • Target information above from: UniProt accession O60543 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12683 Mouse
    • SwissProt: O70302 Mouse
    • Unigene: 449 Mouse
    • Alternative names

      • Cell death activator CIDE A antibody
      • Cell death activator CIDE-A antibody
      • Cell Death Inducing DFFA Like Effector A antibody
      • cell death inducing DNA fragmentation factor, alpha subunit like effector A antibody
      • Cell death-inducing DFFA-like effector A antibody
      • CIDE-A antibody
      • CIDEA antibody
      • CIDEA_HUMAN antibody
      see all

    Images

    • Western blot - Anti-CIDE A antibody (ab8402)
      Western blot - Anti-CIDE A antibody (ab8402)
      Anti-CIDE A antibody (ab8402) at 1/500 dilution + Murine heart tissue lysate

      Predicted band size: 25 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CIDE A antibody (ab8402)
      Immunocytochemistry/ Immunofluorescence - Anti-CIDE A antibody (ab8402)
      Immunofluorescence of CIDE-A in Mouse Heart cells using ab8402 at 20 ug/ml.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab8402? Please let us know so that we can cite the reference in this datasheet.

    ab8402 has been referenced in 9 publications.

    • Zhou YP  et al. Adenosine A2A receptor activation prevents DOCA-salt induced hypertensive cardiac remodeling via iBAT. Biochem Biophys Res Commun N/A:N/A (2020). PubMed: 32087969
    • Liu W  et al. Stat5a promotes brown adipocyte differentiation and thermogenic program through binding and transactivating the Kdm6a promoter. Cell Cycle 19:895-905 (2020). PubMed: 32207362
    • Takahashi J  et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
    • Zhou J  et al. Liraglutide induces beige fat development and promotes mitochondrial function in diet induced obesity mice partially through AMPK-SIRT-1-PGC1-a cell signaling pathway. Endocrine N/A:N/A (2018). PubMed: 30535743
    • He Q  et al. SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice. Sci Rep 8:1200 (2018). PubMed: 29352167
    • Ruan CC  et al. A2A Receptor Activation Attenuates Hypertensive Cardiac Remodeling via Promoting Brown Adipose Tissue-Derived FGF21. Cell Metab 28:476-489.e5 (2018). PubMed: 30017353
    • Zhang YY  et al. Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance Through ERa/HGF/Met Pathway. Diabetes 66:1535-1547 (2017). PubMed: 28325853
    • Binder AK  et al. Expression of Human NSAID Activated Gene 1 in Mice Leads to Altered Mammary Gland Differentiation and Impaired Lactation. PLoS One 11:e0146518 (2016). PubMed: 26745373
    • Carr SK  et al. Maternal diet amplifies the hepatic aging trajectory of Cidea in male mice and leads to the development of fatty liver. FASEB J 28:2191-201 (2014). PubMed: 24481968

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