Anti-CIDE A antibody (ab8402)
- Datasheet
- References (5)
- Protocols
Overview
-
Product nameAnti-CIDE A antibody
See all CIDE A primary antibodies -
DescriptionRabbit polyclonal to CIDE A
-
Host speciesRabbit
-
SpecificityDoes not recognise CIDE-B.
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Mouse
-
Immunogen
Synthetic peptide, corresponding to amino acids 200-214 of Mouse CIDE A.
-
Positive control
- Tissue lysate of murine heart. An approximately 25 kDa band can be detected.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C.
-
Storage bufferPreservative: 0.02% Sodium azide
-
Concentration information loading... -
PurityAffinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Light chain typeunknown
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab8402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 25 kDa.Can be blocked with CIDE A peptide (ab8434). | |
| ICC/IF | Use a concentration of 20 µg/ml. |
Target
-
RelevanceApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICARD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B (for cell death inducing DFF like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE A is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE A is expressed in many tissues.
-
Cellular localizationNuclear. Cytoplasm; mitochondrial envelope; lipid droplet.
-
Database links
- Entrez Gene: 12683 Mouse
- SwissProt: O70302 Mouse
- Unigene: 449 Mouse
-
Alternative names
- Cell death activator CIDE A antibody
- Cell Death Inducing DFFA Like Effector A antibody
- cell death inducing DNA fragmentation factor, alpha subunit like effector A antibody
see all
Images
-
Western blot - Anti-CIDE A antibody (ab8402)Anti-CIDE A antibody (ab8402) at 1/500 dilution + Murine heart tissue lysate
Predicted band size: 25 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CIDE A antibody (ab8402)Immunofluorescence of CIDE-A in Mouse Heart cells using ab8402 at 20 ug/ml.
Datasheets and documents
References
This product has been referenced in:
- He Q et al. SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice. Sci Rep 8:1200 (2018). Read more (PubMed: 29352167) »
- Ruan CC et al. A2A Receptor Activation Attenuates Hypertensive Cardiac Remodeling via Promoting Brown Adipose Tissue-Derived FGF21. Cell Metab 28:476-489.e5 (2018). Read more (PubMed: 30017353) »
