Anti-CIDE A antibody (ab8402)
Key features and details
- Rabbit polyclonal to CIDE A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CIDE A antibody
See all CIDE A primary antibodies -
Description
Rabbit polyclonal to CIDE A -
Host species
Rabbit -
Specificity
Does not recognise CIDE-B. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
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Positive control
- WB: Mouse heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 25 kDa.
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| ICC/IF |
Use a concentration of 20 µg/ml.
|
| Notes |
|---|
|
WB
1/500. Predicted molecular weight: 25 kDa. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear. -
Tissue specificity
Expressed in omental and subcutaneous adipose tissue (at protein level). -
Involvement in disease
In omental and subcutaneous adipose tissue of obese patients matched for BMI, expression levels correlate with insulin sensitivity. Expression is increased 5-6 fold in the group of patients with high insulin sensitivity, compared to the insulin-resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle. -
Sequence similarities
Contains 1 CIDE-N domain. -
Cellular localization
Lipid droplet. Nucleus. Enriched at lipid droplet contact sites. Has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed (PubMed:18509062). - Information by UniProt
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Database links
- Entrez Gene: 12683 Mouse
- SwissProt: O70302 Mouse
- Unigene: 449 Mouse
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Alternative names
- Cell death activator CIDE A antibody
- Cell death activator CIDE-A antibody
- Cell Death Inducing DFFA Like Effector A antibody
see all
Images
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Western blot - Anti-CIDE A antibody (ab8402)Anti-CIDE A antibody (ab8402) at 1/500 dilution + Murine heart tissue lysate
Predicted band size: 25 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CIDE A antibody (ab8402)Immunofluorescence of CIDE-A in Mouse Heart cells using ab8402 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab8402 has been referenced in 9 publications.
- Zhou YP et al. Adenosine A2A receptor activation prevents DOCA-salt induced hypertensive cardiac remodeling via iBAT. Biochem Biophys Res Commun N/A:N/A (2020). PubMed: 32087969
- Liu W et al. Stat5a promotes brown adipocyte differentiation and thermogenic program through binding and transactivating the Kdm6a promoter. Cell Cycle 19:895-905 (2020). PubMed: 32207362
- Takahashi J et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
- Zhou J et al. Liraglutide induces beige fat development and promotes mitochondrial function in diet induced obesity mice partially through AMPK-SIRT-1-PGC1-a cell signaling pathway. Endocrine N/A:N/A (2018). PubMed: 30535743
- He Q et al. SREBP1c mediates the effect of acetaldehyde on Cidea expression in Alcoholic fatty liver Mice. Sci Rep 8:1200 (2018). PubMed: 29352167
- Ruan CC et al. A2A Receptor Activation Attenuates Hypertensive Cardiac Remodeling via Promoting Brown Adipose Tissue-Derived FGF21. Cell Metab 28:476-489.e5 (2018). PubMed: 30017353
- Zhang YY et al. Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance Through ERa/HGF/Met Pathway. Diabetes 66:1535-1547 (2017). PubMed: 28325853
- Binder AK et al. Expression of Human NSAID Activated Gene 1 in Mice Leads to Altered Mammary Gland Differentiation and Impaired Lactation. PLoS One 11:e0146518 (2016). PubMed: 26745373
- Carr SK et al. Maternal diet amplifies the hepatic aging trajectory of Cidea in male mice and leads to the development of fatty liver. FASEB J 28:2191-201 (2014). PubMed: 24481968