Product nameAnti-Cig2 antibody [CIG 3A11/5]
DescriptionMouse monoclonal [CIG 3A11/5] to Cig2
Tested applicationsSuitable for: WB, ELISA, IPmore details
Species reactivityReacts with: Schizosaccharomyces pombe
Bacterial S. pombe Cig2 purified protein.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Clone numberCIG 3A11/5
Our Abpromise guarantee covers the use of ab10881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
RelevanceCig2 is a fission yeast (Schizosaccharomyces pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (CDK), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.
- B type S phase cyclin antibody
All lanes : Anti-Cig2 antibody [CIG 3A11/5] (ab10881) at 1/1000 dilution
Lane 1 : 20ug untreated S. pombe wildtype cells, whole cell lysate
Lane 2 : 20ug S. pombe wildtype cells 8mM HU treated for 1 hour, whole cell lysate
Lane 3 : 20ug S. pombe wildtype cells 8mM HU treated for 2 hours, whole cell lysate
Lane 4 : 20ug S. pombe wildtype cells 8mM HU treated for 3 hours, whole cell lysate
All lanes : HRP conjugated sheep anti-mouse antibody
Developed using the ECL technique.
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This product has been referenced in:
- Chu Z et al. A Cds1-mediated checkpoint protects the MBF activator Rep2 from ubiquitination by anaphase-promoting complex/cyclosome-Ste9 at S-phase arrest in fission yeast. Mol Cell Biol 29:4959-70 (2009). Read more (PubMed: 19596787) »
- Chu Z et al. Modulation of cell cycle-specific gene expressions at the onset of S phase arrest contributes to the robust DNA replication checkpoint response in fission yeast. Mol Biol Cell 18:1756-67 (2007). Read more (PubMed: 17332498) »