Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by delipidation and defibrination.
Our Abpromise guarantee covers the use of ab7541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 123 kDa.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 17586661
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionEssential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
Involvement in diseaseBare lymphocyte syndrome 2
Sequence similaritiesContains 4 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
DomainThe acetyltransferase domain is necessary for activation of both class I and class II transcription.
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
modificationsAutophosphorylated, affecting interaction with TAF7.
Cellular localizationNucleus. Nucleus, PML body. Recruited to PML body by PML.
- Information by UniProt
- C2TA antibody
- C2TA_HUMAN antibody
- CIITA antibody
This product has been referenced in:
- Christova R et al. P-STAT1 mediates higher-order chromatin remodelling of the human MHC in response to IFNgamma. J Cell Sci 120:3262-70 (2007). WB ; Human . Read more (PubMed: 17726060) »
- Herrmann TL et al. MHC Class II levels and intracellular localization in human dendritic cells are regulated by calmodulin kinase II. J Leukoc Biol 82:686-99 (2007). Flow Cyt . Read more (PubMed: 17586661) »