Product nameCIKS overexpression 293T lysate (whole cell)
DescriptionCIKS overexpression lysate
ab94245 is a 293T cell transfected lysate in which Human CIKS has been transiently over-expressed using a pCMV-CIKS full length plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundFunction: Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. Tissue specificity: Widely expressed. Similarity: Contains 1 SEFIR domain.
Our Abpromise guarantee covers the use of ab94245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94245 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-CIKS/ACT1 antibody (ab56758) at 1/500 dilution
Lane 1 :
CIKS overexpression 293T lysate (whole cell) (ab94245)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : oat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94245 has not yet been referenced specifically in any publications.