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    ciksact1-antibody-ab137395.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH
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Anti-CIKS/ACT1 antibody (ab137395)

  • Datasheet
  • SDS
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Western blot - Anti-CIKS/ACT1 antibody (ab137395)
  • Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab137395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab137395)

Key features and details

  • Rabbit polyclonal to CIKS/ACT1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human CIKS/ACT1 protein (ab161141)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CIKS/ACT1 antibody
    See all CIKS/ACT1 primary antibodies
  • Description

    Rabbit polyclonal to CIKS/ACT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human CIKS/ACT1 aa 371-574.
    Database link: O43734

  • Positive control

    • HepG2 whole cell lysate (ab7900), HeLa cells, Colon ca tissue
  • General notes

     This product was previously labelled as CIKS

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFkB pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CIKS/ACT1 protein (ab161141)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
ICC/IF
1/100 - 1/1000.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 SEFIR domain.
  • Target information above from: UniProt accession O43734 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10758 Human
    • Entrez Gene: 103213 Mouse
    • Entrez Gene: 361857 Rat
    • Omim: 607043 Human
    • SwissProt: O43734 Human
    • Unigene: 561514 Human
    • Alternative names

      • Act 1 antibody
      • ACT1 antibody
      • Activator of cAMP responsive element modulator (CREM) in testis antibody
      • Adapter protein CIKS antibody
      • C6ORF4 antibody
      • C6ORF5 antibody
      • C6ORF6 antibody
      • Chromosome 6 Open Reading Frame 4 antibody
      • Chromosome 6 Open Reading Frame 5 antibody
      • Chromosome 6 Open Reading Frame 6 antibody
      • CIKS_HUMAN antibody
      • Connection to IKK and SAPK / JNK antibody
      • Connection to IKK and SAPK/JNK antibody
      • Nuclear Factor Kappa B Activator 1 antibody
      • Nuclear factor NF kappa B activator 1 antibody
      • Nuclear factor NF-kappa-B activator 1 antibody
      • TRAF3 interacting protein 2 antibody
      • TRAF3-interacting protein 2 antibody
      • TRAF31P2 antibody
      • TRAF3IP2 antibody
      see all

    Images

    • Western blot - Anti-CIKS/ACT1 antibody (ab137395)
      Western blot - Anti-CIKS/ACT1 antibody (ab137395)
      Anti-CIKS/ACT1 antibody (ab137395) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg

      Predicted band size: 65 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab137395)
      Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab137395)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelled with ab137395 at 1/200 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab137395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab137395)
      Immunohistochemical analysis of paraffin embedded Colon ca tissue labelled with ab137395 at 1/250 dilution.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab137395? Please let us know so that we can cite the reference in this datasheet.

    ab137395 has not yet been referenced specifically in any publications.

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