Anti-CIKS/ACT1 antibody (ab137395)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CIKS/ACT1 antibody
See all CIKS/ACT1 primary antibodies -
Description
Rabbit polyclonal to CIKS/ACT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human CIKS/ACT1 aa 371-574.
Database link: O43734 -
Positive control
- HepG2 whole cell lysate (ab7900), HeLa cells, Colon ca tissue
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General notes
This product was previously labelled as CIKS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 65 kDa. | |
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 SEFIR domain. - Information by UniProt
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Database links
- Entrez Gene: 10758 Human
- Entrez Gene: 103213 Mouse
- Entrez Gene: 361857 Rat
- Omim: 607043 Human
- SwissProt: O43734 Human
- Unigene: 561514 Human
- Unigene: 436686 Mouse
- Unigene: 9031 Rat
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Alternative names
- Act 1 antibody
- ACT1 antibody
- Activator of cAMP responsive element modulator (CREM) in testis antibody
see all
Images
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Western blot - Anti-CIKS/ACT1 antibody (ab137395)Anti-CIKS/ACT1 antibody (ab137395) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (ab137395)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelled with ab137395 at 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (ab137395)Immunohistochemical analysis of paraffin embedded Colon ca tissue labelled with ab137395 at 1/250 dilution.
Protocols
References
ab137395 has not yet been referenced specifically in any publications.
