Overview

  • Product name

  • Description

    Rabbit polyclonal to Cingulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 200-250 of Human Cingulin (NP_065821.1).

  • Positive control

    • 293T, Jurkat, and HeLa whole cell lysate (ab150035)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
  • Tissue specificity

    Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
  • Sequence similarities

    Belongs to the cingulin family.
  • Domain

    Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
  • Cellular localization

    Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6330408J11Rik antibody
    • AI647528 antibody
    • AI987749 antibody
    • cgn antibody
    • CING_HUMAN antibody
    • Cingulin antibody
    • DKFZp779N1112 antibody
    • FLJ39281 antibody
    • KIAA1319 antibody
    • MGC118157 antibody
    see all

Images

  • All lanes : Anti-Cingulin antibody (ab117796) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg/ml
    Lane 2 : 293T whole cell lysate at 15 µg/ml
    Lane 3 : Jurkat whole cell lysate at 50 µg/ml
    Lane 4 : HeLa whole cell lysate at 50 µg/ml

    Developed using the ECL technique.

    Predicted band size: 136 kDa


    Exposure time: 10 seconds
  • ab117796 at 1 µg/ml staining Cingulin in 293T cell lysate immunoprecipitated using ab117796 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds

References

ab117796 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab117796.
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