Key features and details
- Rabbit polyclonal to Cingulin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab238116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
FunctionProbably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Tissue specificityLocalized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
Sequence similaritiesBelongs to the cingulin family.
DomainDeletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
Cellular localizationCell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
- Information by UniProt
- 6330408J11Rik antibody
- AI647528 antibody
- AI987749 antibody
Paraffin-embedded human cervical cancer tissue stained for Cingulin using ab238116 at 1/800 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238116 has not yet been referenced specifically in any publications.