Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14445] to CINP - N-terminal
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-CINP antibody [EPR14445] - N-terminal
See all CINP primary antibodies
DescriptionRabbit monoclonal [EPR14445] to CINP - N-terminal
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CINP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9BW66
- 293, Jurkat, and HeLa lysate. HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab183519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).|
|IP||1/30 - 1/50.|
FunctionInteracts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling.
Sequence similaritiesBelongs to the CINP family.
modificationsPhosphorylated by CDC7 but not by CDK2.
Cellular localizationNucleus. Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication.
- Information by UniProt
- CDK2-interacting protein antibody
- CINP antibody
- CINP protein antibody
All lanes : Anti-CINP antibody [EPR14445] - N-terminal (ab183519) at 1/1000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
Immunofluorescent analysis of 20°C Acetone fixed HeLa cells labeling CINP with ab183519 at 1/100 dilution (green). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Right panel is counterstained with Dapi (blue).
Western blot analysis of CINP in immunoprecipitation pellets from 293 lysate.
Lane 1: ab183159 used at a 1/50 dilution.
Lane 2: TBS negative control.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
ab183519 has not yet been referenced specifically in any publications.