Recombinant
RabMAb

Recombinant Anti-CINP antibody [EPR14445] - N-terminal (ab183519)

Overview

  • Product name

    Anti-CINP antibody [EPR14445] - N-terminal
    See all CINP primary antibodies
  • Description

    Rabbit monoclonal [EPR14445] to CINP - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CINP aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9BW66

  • Positive control

    • 293, Jurkat, and HeLa lysate. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14445
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).
ICC/IF 1/100.
IP 1/30 - 1/50.

Target

  • Function

    Interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling.
  • Sequence similarities

    Belongs to the CINP family.
  • Post-translational
    modifications

    Phosphorylated by CDC7 but not by CDK2.
  • Cellular localization

    Nucleus. Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDK2-interacting protein antibody
    • CINP antibody
    • CINP protein antibody
    • CINP_HUMAN antibody
    • Cyclin dependent kinase 2 interacting protein antibody
    • Cyclin-dependent kinase 2-interacting protein antibody
    • MGC849 antibody
    see all

Images

  • All lanes : Anti-CINP antibody [EPR14445] - N-terminal (ab183519) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunofluorescent analysis of 20°C Acetone fixed HeLa cells labeling CINP with ab183519 at 1/100 dilution (green). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Right panel is counterstained with Dapi (blue).

  • Western blot analysis of CINP in immunoprecipitation pellets from 293 lysate.

    Lane 1: ab183159 used at a 1/50 dilution.

    Lane 2: TBS negative control.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.

References

ab183519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab183519.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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