Recombinant Anti-CINP antibody [EPR14445] - N-terminal (ab183519)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14445] to CINP - N-terminal
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CINP antibody [EPR14445] - N-terminal
See all CINP primary antibodies -
Description
Rabbit monoclonal [EPR14445] to CINP - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, Jurkat, and HeLa lysate. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14445 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).
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ICC/IF |
1/100.
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IP |
1/30 - 1/50.
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Notes |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). |
ICC/IF
1/100. |
IP
1/30 - 1/50. |
Target
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Function
Interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling. -
Sequence similarities
Belongs to the CINP family. -
Post-translational
modificationsPhosphorylated by CDC7 but not by CDK2. -
Cellular localization
Nucleus. Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication. - Information by UniProt
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Database links
- Entrez Gene: 51550 Human
- Entrez Gene: 67236 Mouse
- Entrez Gene: 299334 Rat
- Omim: 613362 Human
- SwissProt: Q9BW66 Human
- SwissProt: Q9D0V8 Mouse
- Unigene: 129634 Human
- Unigene: 287157 Mouse
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Alternative names
- CDK2-interacting protein antibody
- CINP antibody
- CINP protein antibody
see all
Images
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All lanes : Anti-CINP antibody [EPR14445] - N-terminal (ab183519) at 1/1000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
Immunofluorescent analysis of 20°C Acetone fixed HeLa cells labeling CINP with ab183519 at 1/100 dilution (green). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Right panel is counterstained with Dapi (blue).
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Western blot analysis of CINP in immunoprecipitation pellets from 293 lysate.
Lane 1: ab183159 used at a 1/50 dilution.
Lane 2: TBS negative control.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab183519 has been referenced in 2 publications.
- Ni C et al. Labeling of heterochronic ribosomes reveals C1ORF109 and SPATA5 control a late step in human ribosome assembly. Cell Rep 38:110597 (2022). PubMed: 35354024
- Wu Q et al. CINP is a novel cofactor of KLF5 required for its role in the promotion of cell proliferation, survival and tumor growth. Int J Cancer 144:582-594 (2019). PubMed: 30289973