Product nameAnti-CINP antibody [EPR14446]
See all CINP primary antibodies
DescriptionRabbit monoclonal [EPR14446] to CINP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CINP aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q9BW66
- Jurkat, HeLa, 293 and MCF7 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).|
FunctionInteracts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling.
Sequence similaritiesBelongs to the CINP family.
modificationsPhosphorylated by CDC7 but not by CDK2.
Cellular localizationNucleus. Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication.
- Information by UniProt
- CDK2-interacting protein antibody
- CINP antibody
- CINP protein antibody
Western Blot analysis of 293 cell lysate immunoprecipitated with ab180955 at 1/60 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/500 dilution.
All lanes : Anti-CINP antibody [EPR14446] (ab180955) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
ab180955 has not yet been referenced specifically in any publications.