Key features and details
- Rabbit polyclonal to CIP29
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CIP29 antibody
See all CIP29 primary antibodies
DescriptionRabbit polyclonal to CIP29
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Orangutan, Xenopus tropicalis
Synthetic peptide within Human CIP29 aa 160-210. The exact sequence is proprietary. (NP_149073.1).
Database link: P82979
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225694 was affinity purified using an epitope specific to CIP29 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 24 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis.
Tissue specificityLow expression in spleen, liver, pancreas, testis, thymus, heart, and kidney. Increased levels are seen in hepatocellular carcinoma and pancreatic adenocarcinoma.
Sequence similaritiesContains 1 SAP domain.
- Information by UniProt
- Cytokine induced protein of 29 kDa antibody
- Cytokine-induced protein of 29 kDa antibody
- HCC 1 antibody
All lanes : Anti-CIP29 antibody (ab225694)
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 30 seconds
CIP29 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225694 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225694 at 0.4 µg/ml.
Lane 1: ab225694 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225694 has not yet been referenced specifically in any publications.