Overview

  • Product name

    Anti-CIP2A antibody [2A1-9A4]
    See all CIP2A primary antibodies
  • Description

    Mouse monoclonal [2A1-9A4] to CIP2A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CIP2A (N terminal).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.
  • General notes

    Protein previously labeled as p90 Autoantigen.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA

    pH 7-8
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    The antibody was purified using a mouse immunoglobulin binding ligand.
  • Clonality

    Monoclonal
  • Clone number

    2A1-9A4
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/25000. Predicted molecular weight: 102 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • Tissue specificity

    Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
  • Cellular localization

    Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancerous inhibitor of PP2A antibody
    • Cancerous Inhibitor of Protein Phosphatase 2A antibody
    • CIP2A antibody
    • CIP2A_HUMAN antibody
    • FLJ12850 antibody
    • Kiaa1524 antibody
    • MGC163436 antibody
    • p90 antibody
    • p90 autoantigen antibody
    • Protein CIP2A antibody
    see all

Images

  • All lanes : Anti-CIP2A antibody [2A1-9A4] (ab128179) at 0.04 µg

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 102 kDa


    Exposure time: 10 seconds
  • 293T whole cell lysate (1 mg for IP, 20% of IP loaded) was used for immunoprecipitation with ab128179 at 3 µg/mg (lane 1) and control IgG (lane 2). Western blotting was performed using ab128179 at 0.04 µg/ml. Detection by chemiluminescence was performed for 10 seconds.

References

This product has been referenced in:

  • Wei R  et al. MicroRNA-218 inhibits the cell proliferation and migration in clear cell renal cell carcinoma through targeting cancerous inhibitor of protein phosphatase 2A. Oncol Lett 17:3211-3218 (2019). Read more (PubMed: 30867751) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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