Key features and details
- Mouse monoclonal [2A1-9A4] to CIP2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CIP2A antibody [2A1-9A4]
See all CIP2A primary antibodies
DescriptionMouse monoclonal [2A1-9A4] to CIP2A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CIP2A (N terminal).
- HeLa, 293T and Jurkat whole cell lysates.
Protein previously labeled as p90 Autoantigen.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Purification notesThe antibody was purified using a mouse immunoglobulin binding ligand.
Light chain typekappa
Our Abpromise guarantee covers the use of ab128179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/25000. Predicted molecular weight: 102 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionOncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
Tissue specificityExpressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
Cellular localizationMembrane. Cytoplasm. Slightly concentrates in the perinuclear region.
- Information by UniProt
- Cancerous inhibitor of PP2A antibody
- Cancerous Inhibitor of Protein Phosphatase 2A antibody
- CIP2A antibody
All lanes : Anti-CIP2A antibody [2A1-9A4] (ab128179) at 0.04 µg
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 10 seconds
293T whole cell lysate (1 mg for IP, 20% of IP loaded) was used for immunoprecipitation with ab128179 at 3 µg/mg (lane 1) and control IgG (lane 2). Western blotting was performed using ab128179 at 0.04 µg/ml. Detection by chemiluminescence was performed for 10 seconds.
ab128179 has been referenced in 1 publication.
- Wei R et al. MicroRNA-218 inhibits the cell proliferation and migration in clear cell renal cell carcinoma through targeting cancerous inhibitor of protein phosphatase 2A. Oncol Lett 17:3211-3218 (2019). PubMed: 30867751